Cell-repellant Dextran Coatings of Porous Titania Using Mussel Adhesion Chemistry

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The resistance of bioceramics against non-specific adsorption of serum proteins is critical for a wide range of biomedical applications. Some polysaccharides serve as natural protein-resistant molecules in extracellular matrices; however, the stable adhesion of polysaccharides to ceramic biomaterials in an aqueous solution is very challenging because chemical linkages at organic/inorganic interfaces are susceptible to hydrolytic degradation. Here, a catechol-grafted dextran, which strongly binds to titania (TiO2) in an aqueous milieu to effectively suppress cell adhesion through anti-fouling activity against non-specific protein adsorption, is introduced. Catechol is conjugated approximately to 6.7 mol% of glucose units of dextran via a carbamate ester linkage, corresponding to roughly three catechols per dextran chain having an average molecular weight of 6kDa. Multivalent interactions of catechols with a titanium atom, enabled by the graft-type structure, provide a very stable coating of dextran on this inorganic surface. The adhesion of HeLa cells on the dextran-coated titania surface is reduced by 2.4-fold compared to that on a pristine titania surface. These results suggest that the graft-type incorporation of a small number of catechol moieties along a dextran backbone is an effective means of producing a stable anti-fouling interface on inorganic biomaterials in an aqueous environment.
Publisher
WILEY-V C H VERLAG GMBH
Issue Date
2013-11
Language
English
Article Type
Article
Citation

MACROMOLECULAR BIOSCIENCE, v.13, no.11, pp.1511 - 1519

ISSN
1616-5187
DOI
10.1002/mabi.201300224
URI
http://hdl.handle.net/10203/188746
Appears in Collection
MS-Journal Papers(저널논문)CH-Journal Papers(저널논문)
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