Simple super-resolution live-cell imaging based on diffusion-assisted Forster resonance energy transfer (AH) 장재덕-BK포닥

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Despite the recent development of several super-resolution fluorescence microscopic techniques, there are still few techniques that can be readily employed in conventional imaging systems. We present a very simple, rapid, general and cost-efficient super-resolution imaging method, which can be directly employed in a simple fluorescent imaging system with general fluorophores. Based on diffusion-assisted Forster resonance energy transfer (FRET), fluorescent donor molecules that label specific target structures can be stochastically quenched by diffusing acceptor molecules, thereby temporally separating otherwise spatially overlapped fluorescence signals and allowing super-resolution imaging. The proposed method provides two-to three-fold-enhancement in spatial resolution, a significant optical sectioning property, and favorable temporal resolution in live-cell imaging. We demonstrate super-resolution live-cell dynamic imaging using general fluorophores in a standard epi-fluorescence microscope with light-emitting diode (LED) illumination. Due to the simplicity of this approach, we expect that the proposed method will prove an attractive option for super-resolution imaging.
Publisher
NATURE PUBLISHING GROUP
Issue Date
2013-02
Language
English
Article Type
Article
Citation

SCIENTIFIC REPORTS, v.3, pp.1208

ISSN
2045-2322
DOI
10.1038/srep01208
URI
http://hdl.handle.net/10203/242217
Appears in Collection
PH-Journal Papers(저널논문)BiS-Journal Papers(저널논문)CH-Journal Papers(저널논문)BS-Journal Papers(저널논문)
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