Posttranscriptional Crossregulation between Drosha and DGCR8

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The Drosha-DGCR8 complex, also known as Microprocessor, is essential for microRNA (miRNA) maturation. Drosha functions as the catalytic subunit, while DGCR8 (also known as Pasha) recognizes the RNA substrate. Although the action mechanism of this complex has been intensively studied, it remains unclear how Drosha and DGCR8 are regulated and if these proteins have any additional role(s) apart from miRNA processing. Here, we report that Drosha and DGCR8 regulate each other posttranscriptionally. The Drosha-DGCR8 complex cleaves the hairpin structures embedded in the DGCR8 mRNA and thereby destabilizes the mRNA. We further find that DGCR8 stabilizes the Drosha protein via protein-protein interaction. This crossregulation between Drosha and DGCR8 may contribute to the homeostatic control of miRNA biogenesis. Furthermore, microarray analyses suggest that a number of mRNAs may be downregulated in a Microprocessor-dependent, miRNA-independent manner. Our study reveals a previously unsuspected function of Microprocessor in mRNA stability control.
Publisher
CELL PRESS
Issue Date
2009-01
Language
English
Article Type
Article
Keywords

MICROPROCESSOR COMPLEX; MICRORNA BIOGENESIS; ALZHEIMERS-DISEASE; RNA INTERFERENCE; EXPRESSION; PROLIFERATION; DROSOPHILA; MIR-222; PATHWAY; CELLS

Citation

CELL, v.136, no.1, pp.75 - 84

ISSN
0092-8674
DOI
10.1016/j.cell.2008.10.053
URI
http://hdl.handle.net/10203/221043
Appears in Collection
MSE-Journal Papers(저널논문)
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