Targeted and high-throughput gene knockdown in diverse bacteria using synthetic sRNAs

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dc.contributor.authorCho, Jae Sungko
dc.contributor.authorYang, Dongsooko
dc.contributor.authorPrabowo, Cindy Pricilia Suryako
dc.contributor.authorGhiffary, Mohammad Rifqiko
dc.contributor.authorHan, Taeheeko
dc.contributor.authorChoi, Kyeong Rokko
dc.contributor.authorMoon, Cheonwooko
dc.contributor.authorZhou, Hengruiko
dc.contributor.authorRyu, Jae Yongko
dc.contributor.authorKim, Hyun Ukko
dc.contributor.authorLee, Sang Yupko
dc.date.accessioned2023-05-12T02:01:14Z-
dc.date.available2023-05-12T02:01:14Z-
dc.date.created2023-05-08-
dc.date.created2023-05-08-
dc.date.issued2023-04-
dc.identifier.citationNATURE COMMUNICATIONS, v.14, no.1-
dc.identifier.issn2041-1723-
dc.identifier.urihttp://hdl.handle.net/10203/306749-
dc.description.abstractSynthetic sRNAs allow knockdown of target genes at translational level, but have been restricted to a limited number of bacteria. Here, we report the development of a broad-host-range synthetic sRNA (BHR-sRNA) platform employing the RoxS scaffold and the Hfq chaperone from Bacillus subtilis. BHR-sRNA is tested in 16 bacterial species including commensal, probiotic, pathogenic, and industrial bacteria, with >50% of target gene knockdown achieved in 12 bacterial species. For medical applications, virulence factors in Staphylococcus epidermidis and Klebsiella pneumoniae are knocked down to mitigate their virulence-associated phenotypes. For metabolic engineering applications, high performance Corynebacterium glutamicum strains capable of producing valerolactam (bulk chemical) and methyl anthranilate (fine chemical) are developed by combinatorial knockdown of target genes. A genome-scale sRNA library covering 2959 C. glutamicum genes is constructed for high-throughput colorimetric screening of indigoidine (natural colorant) overproducers. The BHR-sRNA platform will expedite engineering of diverse bacteria of both industrial and medical interest.-
dc.languageEnglish-
dc.publisherNATURE PORTFOLIO-
dc.titleTargeted and high-throughput gene knockdown in diverse bacteria using synthetic sRNAs-
dc.typeArticle-
dc.identifier.wosid001002170200025-
dc.identifier.scopusid2-s2.0-85153687205-
dc.type.rimsART-
dc.citation.volume14-
dc.citation.issue1-
dc.citation.publicationnameNATURE COMMUNICATIONS-
dc.identifier.doi10.1038/s41467-023-38119-y-
dc.contributor.localauthorChoi, Kyeong Rok-
dc.contributor.localauthorKim, Hyun Uk-
dc.contributor.localauthorLee, Sang Yup-
dc.description.isOpenAccessN-
dc.type.journalArticleArticle-
dc.subject.keywordPlusSTAPHYLOCOCCUS-EPIDERMIDIS-
dc.subject.keywordPlusESCHERICHIA-COLI-
dc.subject.keywordPlusREGULATOR-
dc.subject.keywordPlusHFQ-
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