Effect of serum on the production of foreign protein using vaccinia virus = 혈청이 Vaccinia virus의 외래 단백질 생산에 미치는 영향

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The effect of serum on recombinant protein production using vaccinia virus expression system was studied in both monolayer and suspension cultures of HeLa S3 cells. A model heterologous protein was $\beta$-galactosidase. Calf and horse sera were used, and their concentrations were 0.5-10\%(v/v). In monolayer culture, calf serum did not show any significant influence on $\beta$-galactosidase production. Horse serum, on the other hand, resulted in higher amount of product at 10\% concentration and its level increased continuously up to 96 hr p.i. Maximum total $\beta$-galactosidase values were $3.6 \pm 0.7 I.U./mL$ for calf serum and $2.8 \pm 0.7 I.U./mL$ for horse serum. In suspension culture, both serum showed similar pattern of their own as in monolayer culture. Maximum enzyme levels were 3.3 I.U./mL for calf serum and 3.6 I.U./mL for horse serum. Both sera showed lower maximum value, compared with monolayer culture. Cell numbers were also lower in suspension culture. However, enzyme activity per million cells were about 1.5-fold higher than that in the monolayer culture.
Advisors
Lee, Gyun-Minresearcher이균민researcher
Description
한국과학기술원 : 생물공학과,
Publisher
한국과학기술원
Issue Date
1995
Identifier
98707/325007 / 000933441
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물공학과, 1995.2, [ v, 41 p. ]

URI
http://hdl.handle.net/10203/28473
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=98707&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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