DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Lee, Gyun-Min | - |
dc.contributor.advisor | 이균민 | - |
dc.contributor.author | Jun, Seung-Chul | - |
dc.contributor.author | 전승철 | - |
dc.date.accessioned | 2011-12-12T09:00:31Z | - |
dc.date.available | 2011-12-12T09:00:31Z | - |
dc.date.issued | 1995 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=98707&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/28473 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 생물공학과, 1995.2, [ v, 41 p. ] | - |
dc.description.abstract | The effect of serum on recombinant protein production using vaccinia virus expression system was studied in both monolayer and suspension cultures of HeLa S3 cells. A model heterologous protein was $\beta$-galactosidase. Calf and horse sera were used, and their concentrations were 0.5-10\%(v/v). In monolayer culture, calf serum did not show any significant influence on $\beta$-galactosidase production. Horse serum, on the other hand, resulted in higher amount of product at 10\% concentration and its level increased continuously up to 96 hr p.i. Maximum total $\beta$-galactosidase values were $3.6 \pm 0.7 I.U./mL$ for calf serum and $2.8 \pm 0.7 I.U./mL$ for horse serum. In suspension culture, both serum showed similar pattern of their own as in monolayer culture. Maximum enzyme levels were 3.3 I.U./mL for calf serum and 3.6 I.U./mL for horse serum. Both sera showed lower maximum value, compared with monolayer culture. Cell numbers were also lower in suspension culture. However, enzyme activity per million cells were about 1.5-fold higher than that in the monolayer culture. | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.title | Effect of serum on the production of foreign protein using vaccinia virus | - |
dc.title.alternative | 혈청이 Vaccinia virus의 외래 단백질 생산에 미치는 영향 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 98707/325007 | - |
dc.description.department | 한국과학기술원 : 생물공학과, | - |
dc.identifier.uid | 000933441 | - |
dc.contributor.localauthor | Lee, Gyun-Min | - |
dc.contributor.localauthor | 이균민 | - |
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