Microfluidic dissociation and clearance of Alzheimer's beta-amyloid aggregates

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The abnormal aggregation of beta-amyloid peptide (A beta) in the brain is a major histopathological feature of Alzheimer's disease. Herein, we first report on microfluidic dissociation and clearance analysis of preformed A beta aggregates for parallel screening of aggregate destabilizers. As a proof of the concept for the microfluidic platform, we investigated (1) microfluidics-based clearance of metal ion-induced A beta aggregates using different types of metal chelators, (2) the clearance effect of deferoxamine on A beta aggregates within microchannels, (3) comparison between destabilized A beta dissociated from pre-formed A beta aggregates and remaining deposits within the microchannels both before and after the clearance, and (4) secondary structure change in A beta deposits by the clearance treatment. The microfluidics-based clearance system should be suitable for efficient screening of chemical candidates to enhance the clearance of A beta deposits prior to their in vivo evaluation. (C) 2010 Elsevier Ltd. All rights reserved.
Publisher
Elsevier Sci Ltd
Issue Date
2010-06
Language
English
Article Type
Article
Keywords

HIGH-THROUGHPUT ANALYSIS; FIBRIL FORMATION; IN-VITRO; DISEASE; PEPTIDE; DEPOSITION; TOXICITY; PATHWAYS; ALUMINUM; THERAPY

Citation

BIOMATERIALS, v.31, no.26, pp.6789 - 6795

ISSN
0142-9612
URI
http://hdl.handle.net/10203/25503
Appears in Collection
MS-Journal Papers(저널논문)
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