A Physiology Study of Escherichia coli Overexpressing Phosphoenolpyruvate Carboxykinase
To determine whether intracellular ATP levels can be
affected, Escherichia coli overexpressing phosphoenolpyruvate
carboxykinase (pck) or phosphoenolpyruvate
carboxylase (ppc) were grown in glucose minimal
medium. The Pck-overexpressing cells showed approximately
twice the intracellular ATP concentration, with
22% slower growth than the Ppc-overexpressing strain.
This unexpected result of higher ATP coupled with
slower growth is discussed based on transcriptome
analysis.
- Issue Date
- 2008
- Keywords
PEP carboxykinase; PEP carboxylase; intracellular ATP
- Citation
Bioscience Biotechnology and Biochemistry, Vol.72, No.4, pp.1138-1141
- ISSN
- 0916-8451
- Appears in Collection
- CBE-Journal Papers(저널논문)
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