A Physiology Study of Escherichia coli Overexpressing Phosphoenolpyruvate Carboxykinase

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To determine whether intracellular ATP levels can be affected, Escherichia coli overexpressing phosphoenolpyruvate carboxykinase (pck) or phosphoenolpyruvate carboxylase (ppc) were grown in glucose minimal medium. The Pck-overexpressing cells showed approximately twice the intracellular ATP concentration, with 22% slower growth than the Ppc-overexpressing strain. This unexpected result of higher ATP coupled with slower growth is discussed based on transcriptome analysis.
Publisher
Japan Society for Bioscience, Biotechnology and Agrochemistry
Issue Date
2008
Keywords

PEP carboxykinase; PEP carboxylase; intracellular ATP

Citation

Bioscience Biotechnology and Biochemistry, Vol.72, No.4, pp.1138-1141

ISSN
0916-8451
DOI
10.1271/bbb.70831
URI
http://hdl.handle.net/10203/25254
Appears in Collection
CBE-Journal Papers(저널논문)

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