A Physiology Study of Escherichia coli Overexpressing Phosphoenolpyruvate Carboxykinase

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dc.contributor.authorKwon, Yeong-Deok-
dc.contributor.authorLee, Sang Yup-
dc.contributor.authorKim, Pil-
dc.date.accessioned2011-09-20T02:55:53Z-
dc.date.available2011-09-20T02:55:53Z-
dc.date.issued2008-
dc.identifier.citationBioscience Biotechnology and Biochemistry, Vol.72, No.4, pp.1138-1141en
dc.identifier.issn0916-8451-
dc.identifier.urihttp://hdl.handle.net/10203/25254-
dc.description.abstractTo determine whether intracellular ATP levels can be affected, Escherichia coli overexpressing phosphoenolpyruvate carboxykinase (pck) or phosphoenolpyruvate carboxylase (ppc) were grown in glucose minimal medium. The Pck-overexpressing cells showed approximately twice the intracellular ATP concentration, with 22% slower growth than the Ppc-overexpressing strain. This unexpected result of higher ATP coupled with slower growth is discussed based on transcriptome analysis.en
dc.description.sponsorshipThis work was funded by the Genome-Based Integrated Bioprocess Development Project of the Korean Ministry of Science and Technology. Ms. Kwon was supported in part by a Seoul Metropolitan Scholarship for Science Graduate Students.en
dc.language.isoen_USen
dc.publisherJapan Society for Bioscience, Biotechnology and Agrochemistryen
dc.subjectPEP carboxykinaseen
dc.subjectPEP carboxylaseen
dc.subjectintracellular ATPen
dc.titleA Physiology Study of Escherichia coli Overexpressing Phosphoenolpyruvate Carboxykinaseen
dc.typeArticleen
dc.identifier.doi10.1271/bbb.70831-
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