Biotransformation of oleic acid into 10-ketostearic acid by recombinant Corynebacterium glutamicum-based biocatalyst

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To produce 10-ketostearic acid from oleic acid. Oleic acid was converted to 10-ketostearic acid by a recombinant Corynebacterium glutamicum ATCC 13032 expressing oleate hydratase from Stenotrophomonas maltophilia and a secondary alcohol dehydrogenase from Micrococcus luteus under the control of a synthetic constitutive promoter. Optimal conditions for 10-ketostearic acid production were pH 7.5 and 30 A degrees C with 5 g cells l(-1) and 2.5 g oleic acid l(-1). Under these conditions, the cells produced 1.96 g 10-ketostearic acid l(-1) from oleic acid in 6 h, with a conversion yield of 78 % (w) and a maximum volumetric productivity of 1.67 g l(-1) h(-1). This is the first report of 10-ketostearic acid production using a recombinant C. glutamicum.
Publisher
SPRINGER
Issue Date
2015-05
Language
English
Article Type
Article
Citation

BIOTECHNOLOGY LETTERS, v.37, no.5, pp.1101 - 1106

ISSN
0141-5492
DOI
10.1007/s10529-015-1775-7
URI
http://hdl.handle.net/10203/200747
Appears in Collection
CBE-Journal Papers(저널논문)
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