DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Byeonghun | ko |
dc.contributor.author | Lee, Saebom | ko |
dc.contributor.author | Kim, Hyeonsoo | ko |
dc.contributor.author | Jeong, Kijun | ko |
dc.contributor.author | Park, Jinbyung | ko |
dc.contributor.author | Lee, Eunyeol | ko |
dc.contributor.author | Lee, Jinwon | ko |
dc.date.accessioned | 2015-11-20T07:34:09Z | - |
dc.date.available | 2015-11-20T07:34:09Z | - |
dc.date.created | 2015-05-12 | - |
dc.date.created | 2015-05-12 | - |
dc.date.created | 2015-05-12 | - |
dc.date.issued | 2015-05 | - |
dc.identifier.citation | BIOTECHNOLOGY LETTERS, v.37, no.5, pp.1101 - 1106 | - |
dc.identifier.issn | 0141-5492 | - |
dc.identifier.uri | http://hdl.handle.net/10203/200747 | - |
dc.description.abstract | To produce 10-ketostearic acid from oleic acid. Oleic acid was converted to 10-ketostearic acid by a recombinant Corynebacterium glutamicum ATCC 13032 expressing oleate hydratase from Stenotrophomonas maltophilia and a secondary alcohol dehydrogenase from Micrococcus luteus under the control of a synthetic constitutive promoter. Optimal conditions for 10-ketostearic acid production were pH 7.5 and 30 A degrees C with 5 g cells l(-1) and 2.5 g oleic acid l(-1). Under these conditions, the cells produced 1.96 g 10-ketostearic acid l(-1) from oleic acid in 6 h, with a conversion yield of 78 % (w) and a maximum volumetric productivity of 1.67 g l(-1) h(-1). This is the first report of 10-ketostearic acid production using a recombinant C. glutamicum. | - |
dc.language | English | - |
dc.publisher | SPRINGER | - |
dc.title | Biotransformation of oleic acid into 10-ketostearic acid by recombinant Corynebacterium glutamicum-based biocatalyst | - |
dc.type | Article | - |
dc.identifier.wosid | 000352641700018 | - |
dc.identifier.scopusid | 2-s2.0-84939979965 | - |
dc.type.rims | ART | - |
dc.citation.volume | 37 | - |
dc.citation.issue | 5 | - |
dc.citation.beginningpage | 1101 | - |
dc.citation.endingpage | 1106 | - |
dc.citation.publicationname | BIOTECHNOLOGY LETTERS | - |
dc.identifier.doi | 10.1007/s10529-015-1775-7 | - |
dc.contributor.localauthor | Jeong, Kijun | - |
dc.contributor.nonIdAuthor | Lee, Byeonghun | - |
dc.contributor.nonIdAuthor | Lee, Saebom | - |
dc.contributor.nonIdAuthor | Kim, Hyeonsoo | - |
dc.contributor.nonIdAuthor | Park, Jinbyung | - |
dc.contributor.nonIdAuthor | Lee, Eunyeol | - |
dc.contributor.nonIdAuthor | Lee, Jinwon | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Biocatalysts | - |
dc.subject.keywordAuthor | Biotransformation | - |
dc.subject.keywordAuthor | Corynebacterium glutamicum | - |
dc.subject.keywordAuthor | 10-Ketostearic acid | - |
dc.subject.keywordAuthor | Oleate hydratase | - |
dc.subject.keywordAuthor | Oleic acid | - |
dc.subject.keywordAuthor | Secondary alcohol dehydrogenase | - |
dc.subject.keywordPlus | WHOLE CELLS | - |
dc.subject.keywordPlus | CONVERSION | - |
dc.subject.keywordPlus | EXPRESSION | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.