Study of cellular development and intracellular lipid bodies accumulation in the thraustochytrid Aurantiochytrium sp KRS101

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Transmission electron, confocal microscopy and FACS in conjunction with two different lipophilic fluorescent dyes, BODIPY 505/515 and Nile Red were used to describe the cellular development and lipid bodies formation in Aurantiochytrium sp. KRS101. TEM results revealed that multi-cellular spores were appeared in sporangium during early-exponential phase, and spores were matured in mid-exponential phase followed by release of spores from sporangium in late-exponential phase. TEM and FACS analyses proved that lipid bodies appeared, developed and degenerated in mid-exponential, early- and late-stationary phases, respectively. The staining results in FACS indicate that BODIPY 505/515 is more effective for the vital staining of intracellular lipid bodies than Nile Red. FACS based single cell sorting also showed healthy growth for BODIPY 505/515 stained cells than Nile Red stained cells. In addition, a quantitative baseline was established either for cell growth and/or lipid accumulation based on cell count, fatty acid contents/composition, and sectional/confocal images of KRS101.
Publisher
ELSEVIER SCI LTD
Issue Date
2014-06
Language
English
Article Type
Article
Keywords

DOCOSAHEXAENOIC ACID PRODUCTION; FLOW-CYTOMETRY; CHLAMYDOMONAS-REINHARDTII; BODY FORMATION; NILE RED; MICROALGAE; EXPRESSION; STRAINS; PUFAS; GENE

Citation

BIORESOURCE TECHNOLOGY, v.161, pp.149 - 154

ISSN
0960-8524
DOI
10.1016/j.biortech.2014.03.017
URI
http://hdl.handle.net/10203/189290
Appears in Collection
CBE-Journal Papers(저널논문)
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