DC Field | Value | Language |
---|---|---|
dc.contributor.author | Velmurugan, Natarajan | ko |
dc.contributor.author | Sathishkumar, Yesupatham | ko |
dc.contributor.author | Yim, Sung Sun | ko |
dc.contributor.author | Lee, Yang Soo | ko |
dc.contributor.author | Park, Min S. | ko |
dc.contributor.author | Yang, Ji Won | ko |
dc.contributor.author | Jeong, Ki Jun | ko |
dc.date.accessioned | 2014-09-01T07:34:42Z | - |
dc.date.available | 2014-09-01T07:34:42Z | - |
dc.date.created | 2014-06-10 | - |
dc.date.created | 2014-06-10 | - |
dc.date.created | 2014-06-10 | - |
dc.date.created | 2014-06-10 | - |
dc.date.issued | 2014-06 | - |
dc.identifier.citation | BIORESOURCE TECHNOLOGY, v.161, pp.149 - 154 | - |
dc.identifier.issn | 0960-8524 | - |
dc.identifier.uri | http://hdl.handle.net/10203/189290 | - |
dc.description.abstract | Transmission electron, confocal microscopy and FACS in conjunction with two different lipophilic fluorescent dyes, BODIPY 505/515 and Nile Red were used to describe the cellular development and lipid bodies formation in Aurantiochytrium sp. KRS101. TEM results revealed that multi-cellular spores were appeared in sporangium during early-exponential phase, and spores were matured in mid-exponential phase followed by release of spores from sporangium in late-exponential phase. TEM and FACS analyses proved that lipid bodies appeared, developed and degenerated in mid-exponential, early- and late-stationary phases, respectively. The staining results in FACS indicate that BODIPY 505/515 is more effective for the vital staining of intracellular lipid bodies than Nile Red. FACS based single cell sorting also showed healthy growth for BODIPY 505/515 stained cells than Nile Red stained cells. In addition, a quantitative baseline was established either for cell growth and/or lipid accumulation based on cell count, fatty acid contents/composition, and sectional/confocal images of KRS101. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.title | Study of cellular development and intracellular lipid bodies accumulation in the thraustochytrid Aurantiochytrium sp KRS101 | - |
dc.type | Article | - |
dc.identifier.wosid | 000335436000019 | - |
dc.identifier.scopusid | 2-s2.0-84897470315 | - |
dc.type.rims | ART | - |
dc.citation.volume | 161 | - |
dc.citation.beginningpage | 149 | - |
dc.citation.endingpage | 154 | - |
dc.citation.publicationname | BIORESOURCE TECHNOLOGY | - |
dc.identifier.doi | 10.1016/j.biortech.2014.03.017 | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Yim, Sung Sun | - |
dc.contributor.localauthor | Yang, Ji Won | - |
dc.contributor.localauthor | Jeong, Ki Jun | - |
dc.contributor.nonIdAuthor | Velmurugan, Natarajan | - |
dc.contributor.nonIdAuthor | Sathishkumar, Yesupatham | - |
dc.contributor.nonIdAuthor | Lee, Yang Soo | - |
dc.contributor.nonIdAuthor | Park, Min S. | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Aurantiochytrium sp. | - |
dc.subject.keywordAuthor | TEM analysis | - |
dc.subject.keywordAuthor | BODIPY 505/515 | - |
dc.subject.keywordAuthor | Nile Red | - |
dc.subject.keywordAuthor | Fluorescence-activated cell sorting | - |
dc.subject.keywordPlus | DOCOSAHEXAENOIC ACID PRODUCTION | - |
dc.subject.keywordPlus | FLOW-CYTOMETRY | - |
dc.subject.keywordPlus | CHLAMYDOMONAS-REINHARDTII | - |
dc.subject.keywordPlus | BODY FORMATION | - |
dc.subject.keywordPlus | NILE RED | - |
dc.subject.keywordPlus | MICROALGAE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | STRAINS | - |
dc.subject.keywordPlus | PUFAS | - |
dc.subject.keywordPlus | GENE | - |
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