Identification of Factors Regulating Escherichia coli 2,3-Butanediol Production by Continuous Culture and Metabolic Flux Analysis

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2,3-Butanediol (2,3-BDO) is an organic compound with a wide range of industrial applications. Although Escherichia call is often used for the production of organic compounds, the wild-type E. coil does not contain two essential genes in the 2,3-BDO biosynthesis pathway, and cannot ferment 2,3-BDO. Therefore, a 2,3-BDO biosynthesis mutant strain of Escherichia coil was constructed and cultured. To determine the optimum culture factors for 2,3-BDO production, experiments were conducted under different culture environments ranging from strongly acidic to neutral pH. The extracellular metabolite profiles were obtained using high-performance liquid chromatography (HPLC), and the intracellular metabolite profiles were analyzed by ultra-performance liquid chromatography and quadruple time-of-flight mass spectrometry (UPLC/Q-TOF-MS). Metabolic flux analysis (MFA) was used to integrate these profiles. The metabolite profiles showed that 2,3-BDO production favors an acidic environment (pH 5), whereas cell mass favors a neutral environment. Furthermore, when the pH of the culture fell below 5, both the cell growth and 2,3-BDO production were inhibited.
Publisher
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
Issue Date
2012-05
Language
English
Article Type
Article
Citation

JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.22, no.5, pp.659 - 667

ISSN
1017-7825
DOI
10.4014/jmb.1112.12018
URI
http://hdl.handle.net/10203/104069
Appears in Collection
CBE-Journal Papers(저널논문)
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