DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lu, Mingshou | ko |
dc.contributor.author | Lee, Soojin | ko |
dc.contributor.author | Kim, Borim | ko |
dc.contributor.author | Park, Changhun | ko |
dc.contributor.author | Oh, Minkyu | ko |
dc.contributor.author | Park, Kyungmoon | ko |
dc.contributor.author | Lee, Sang Yup | ko |
dc.contributor.author | Lee, Jinwon | ko |
dc.date.accessioned | 2013-03-13T01:08:38Z | - |
dc.date.available | 2013-03-13T01:08:38Z | - |
dc.date.created | 2012-08-20 | - |
dc.date.created | 2012-08-20 | - |
dc.date.created | 2012-08-20 | - |
dc.date.issued | 2012-05 | - |
dc.identifier.citation | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.22, no.5, pp.659 - 667 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.uri | http://hdl.handle.net/10203/104069 | - |
dc.description.abstract | 2,3-Butanediol (2,3-BDO) is an organic compound with a wide range of industrial applications. Although Escherichia call is often used for the production of organic compounds, the wild-type E. coil does not contain two essential genes in the 2,3-BDO biosynthesis pathway, and cannot ferment 2,3-BDO. Therefore, a 2,3-BDO biosynthesis mutant strain of Escherichia coil was constructed and cultured. To determine the optimum culture factors for 2,3-BDO production, experiments were conducted under different culture environments ranging from strongly acidic to neutral pH. The extracellular metabolite profiles were obtained using high-performance liquid chromatography (HPLC), and the intracellular metabolite profiles were analyzed by ultra-performance liquid chromatography and quadruple time-of-flight mass spectrometry (UPLC/Q-TOF-MS). Metabolic flux analysis (MFA) was used to integrate these profiles. The metabolite profiles showed that 2,3-BDO production favors an acidic environment (pH 5), whereas cell mass favors a neutral environment. Furthermore, when the pH of the culture fell below 5, both the cell growth and 2,3-BDO production were inhibited. | - |
dc.language | English | - |
dc.publisher | KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY | - |
dc.title | Identification of Factors Regulating Escherichia coli 2,3-Butanediol Production by Continuous Culture and Metabolic Flux Analysis | - |
dc.type | Article | - |
dc.identifier.wosid | 000304683100013 | - |
dc.identifier.scopusid | 2-s2.0-84862096503 | - |
dc.type.rims | ART | - |
dc.citation.volume | 22 | - |
dc.citation.issue | 5 | - |
dc.citation.beginningpage | 659 | - |
dc.citation.endingpage | 667 | - |
dc.citation.publicationname | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.identifier.doi | 10.4014/jmb.1112.12018 | - |
dc.contributor.localauthor | Lee, Sang Yup | - |
dc.contributor.nonIdAuthor | Lu, Mingshou | - |
dc.contributor.nonIdAuthor | Lee, Soojin | - |
dc.contributor.nonIdAuthor | Kim, Borim | - |
dc.contributor.nonIdAuthor | Park, Changhun | - |
dc.contributor.nonIdAuthor | Oh, Minkyu | - |
dc.contributor.nonIdAuthor | Park, Kyungmoon | - |
dc.contributor.nonIdAuthor | Lee, Jinwon | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | 2,3-Butanediol fermentation | - |
dc.subject.keywordAuthor | continuous culture | - |
dc.subject.keywordAuthor | pH influence | - |
dc.subject.keywordAuthor | metabolic flux analysis | - |
dc.subject.keywordAuthor | intracellular metabolic measurements | - |
dc.subject.keywordPlus | 1,3-PROPANEDIOL | - |
dc.subject.keywordPlus | DISTRIBUTIONS | - |
dc.subject.keywordPlus | FERMENTATION | - |
dc.subject.keywordPlus | ENTEROBACTER | - |
dc.subject.keywordPlus | KLEBSIELLA | - |
dc.subject.keywordPlus | ACETOIN | - |
dc.subject.keywordPlus | STATE | - |
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