Artificial transcription factors increase production of recombinant antibodies in Chinese hamster ovary cells

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A randomized library that encodes for artificial zinc finger protein transcription factors (ZFP-TF) was constructed and screened for components that increased production of a monoclonal antibody (mAb-72) in Chinese hamster ovary (CHO) cells. One of these ZFP-TF, LK52, increased mAb-72 production similar to 10-fold at similar to 60% transduction efficiency; a mutated version of LK52, however, did not boost mAb-72 production. LK52 also increased production of other mAbs in CHO cells. These results demonstrate that ZFP-TF libraries can be used to identify components that improve antibody production in CHO cells.
Publisher
SPRINGER
Issue Date
2006-01
Language
English
Article Type
Article
Keywords

BUTYRATE-INDUCED APOPTOSIS; MAMMALIAN-CELLS; PROTEIN; CULTURE; EXPRESSION; INDUCTION; LIBRARIES; DNA

Citation

BIOTECHNOLOGY LETTERS, v.28, pp.9 - 15

ISSN
0141-5492
DOI
10.1007/s10529-005-4680-7
URI
http://hdl.handle.net/10203/92574
Appears in Collection
BS-Journal Papers(저널논문)
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