DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kwon, RJ | ko |
dc.contributor.author | Kim, S | ko |
dc.contributor.author | Lee, SI | ko |
dc.contributor.author | Hwang, SJ | ko |
dc.contributor.author | Lee, Gyun-Min | ko |
dc.contributor.author | Kim, JS | ko |
dc.contributor.author | Seol, W | ko |
dc.date.accessioned | 2013-03-08T08:14:47Z | - |
dc.date.available | 2013-03-08T08:14:47Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2006-01 | - |
dc.identifier.citation | BIOTECHNOLOGY LETTERS, v.28, pp.9 - 15 | - |
dc.identifier.issn | 0141-5492 | - |
dc.identifier.uri | http://hdl.handle.net/10203/92574 | - |
dc.description.abstract | A randomized library that encodes for artificial zinc finger protein transcription factors (ZFP-TF) was constructed and screened for components that increased production of a monoclonal antibody (mAb-72) in Chinese hamster ovary (CHO) cells. One of these ZFP-TF, LK52, increased mAb-72 production similar to 10-fold at similar to 60% transduction efficiency; a mutated version of LK52, however, did not boost mAb-72 production. LK52 also increased production of other mAbs in CHO cells. These results demonstrate that ZFP-TF libraries can be used to identify components that improve antibody production in CHO cells. | - |
dc.language | English | - |
dc.publisher | SPRINGER | - |
dc.subject | BUTYRATE-INDUCED APOPTOSIS | - |
dc.subject | MAMMALIAN-CELLS | - |
dc.subject | PROTEIN | - |
dc.subject | CULTURE | - |
dc.subject | EXPRESSION | - |
dc.subject | INDUCTION | - |
dc.subject | LIBRARIES | - |
dc.subject | DNA | - |
dc.title | Artificial transcription factors increase production of recombinant antibodies in Chinese hamster ovary cells | - |
dc.type | Article | - |
dc.identifier.wosid | 000234143800002 | - |
dc.identifier.scopusid | 2-s2.0-29244486764 | - |
dc.type.rims | ART | - |
dc.citation.volume | 28 | - |
dc.citation.beginningpage | 9 | - |
dc.citation.endingpage | 15 | - |
dc.citation.publicationname | BIOTECHNOLOGY LETTERS | - |
dc.identifier.doi | 10.1007/s10529-005-4680-7 | - |
dc.contributor.localauthor | Lee, Gyun-Min | - |
dc.contributor.nonIdAuthor | Kwon, RJ | - |
dc.contributor.nonIdAuthor | Kim, S | - |
dc.contributor.nonIdAuthor | Lee, SI | - |
dc.contributor.nonIdAuthor | Hwang, SJ | - |
dc.contributor.nonIdAuthor | Kim, JS | - |
dc.contributor.nonIdAuthor | Seol, W | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | artificial transcription factors | - |
dc.subject.keywordAuthor | Chinese hamster ovary cells | - |
dc.subject.keywordAuthor | recombinant antibody production | - |
dc.subject.keywordAuthor | zinc finger protein | - |
dc.subject.keywordPlus | BUTYRATE-INDUCED APOPTOSIS | - |
dc.subject.keywordPlus | MAMMALIAN-CELLS | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | CULTURE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | INDUCTION | - |
dc.subject.keywordPlus | LIBRARIES | - |
dc.subject.keywordPlus | DNA | - |
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