Mass production of medium-chain-length poly(3-hydroxyalkanoates) from hydrolyzed corn oil by fed-batch culture of Pseudomonas putida

A novel biopolyester, Medium-Chain-Length Polyhydroxyalkanoates (MCL-PHAs), was made from the corn oil hydrolysate, an inexpensive and renewable carbon source, by a fed-batch culture of Pseudomonas putida with a phosphate limitation. The cell growth, MCL-PHAs accumulation and feeding strategies of corn oil hydrolysate in the cultures of P. putida were investigated in 5 l and 30 l fermentors respectively. In the optimal fermentation, the final cell and MCL-PHAs concentrations reached 103 and 28 g/l, which represents a MCL-PHAs productivity of 0.61 g/l h. It was confirmed by its NMR spectrum that this MCL-PHAs from corn oil hydrolysate contained 4 saturated and 3 unsaturated monomers with a chain length of 6-14 carbon atoms.
Publisher
Springer
Issue Date
2008-12
Language
ENG
Keywords

FATTY-ACIDS; RALSTONIA-EUTROPHA; GLUCOSE; POLY(HYDROXYALKANOATES); POLYHYDROXYALKANOATES; CULTIVATION; YIELD

Citation

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, v.24, no.12, pp.2783 - 2787

ISSN
0959-3993
DOI
10.1007/s11274-008-9808-1
URI
http://hdl.handle.net/10203/87543
Appears in Collection
CBE-Journal Papers(저널논문)
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