Expression, purification and crystallization of recombinant human TRAIL

Cited 15 time in webofscience Cited 0 time in scopus
  • Hit : 258
  • Download : 0
TRAIL (also known as Apo-2L) belongs to the tumour necrosis factor (TNF) cytokine family and induces rapid apoptosis in a wide variety of tumour cell lines upon binding to the death-signalling receptors on the cell membrane. Normal cells are resistant to TRAIL, owing to the expression of decoy receptors which lack functional death domains and antagonize TRAIL-induced apoptosis. Soluble and functional human TRAIL, expressed in Escherichia coli and refolded into a functional form, has been crystallized. The crystals belong to space group P6(3) with unit-cell dimensions a = b = 65.61, c = 131.70 Angstrom. The asymmetric unit contains two molecules of TRAIL, with a crystal volume per protein mass (V-m) of 2.41 Angstrom(3) Da(-1) and a solvent content of about 42% by volume. A native and a platinum-derivative data set to 2.8 and 3.5 Angstrom resolution, respectively, were obtained from frozen crystals. Structure determination by a combined molecular replacement and isomorphous replacement method is in progress.
Publisher
MUNKSGAARD INT PUBL LTD
Issue Date
1999-05
Language
English
Article Type
Article
Keywords

TUMOR-NECROSIS-FACTOR; TNF RECEPTOR SUPERFAMILY; INDUCED CELL-DEATH; FACTOR-ALPHA; CRYSTAL-STRUCTURE; MUTATIONAL ANALYSIS; INDUCED APOPTOSIS; LIGAND; FAMILY; DOMAIN

Citation

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, v.55, pp.1101 - 1104

ISSN
0907-4449
DOI
10.1107/S090744499900164X
URI
http://hdl.handle.net/10203/77612
Appears in Collection
BS-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 15 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0