Expression, purification and crystallization of recombinant human TRAIL

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dc.contributor.authorCha, SSko
dc.contributor.authorShin, HCko
dc.contributor.authorChoi, KYko
dc.contributor.authorOh, Byung-Hako
dc.date.accessioned2013-03-03T06:20:34Z-
dc.date.available2013-03-03T06:20:34Z-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.issued1999-05-
dc.identifier.citationACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, v.55, pp.1101 - 1104-
dc.identifier.issn0907-4449-
dc.identifier.urihttp://hdl.handle.net/10203/77612-
dc.description.abstractTRAIL (also known as Apo-2L) belongs to the tumour necrosis factor (TNF) cytokine family and induces rapid apoptosis in a wide variety of tumour cell lines upon binding to the death-signalling receptors on the cell membrane. Normal cells are resistant to TRAIL, owing to the expression of decoy receptors which lack functional death domains and antagonize TRAIL-induced apoptosis. Soluble and functional human TRAIL, expressed in Escherichia coli and refolded into a functional form, has been crystallized. The crystals belong to space group P6(3) with unit-cell dimensions a = b = 65.61, c = 131.70 Angstrom. The asymmetric unit contains two molecules of TRAIL, with a crystal volume per protein mass (V-m) of 2.41 Angstrom(3) Da(-1) and a solvent content of about 42% by volume. A native and a platinum-derivative data set to 2.8 and 3.5 Angstrom resolution, respectively, were obtained from frozen crystals. Structure determination by a combined molecular replacement and isomorphous replacement method is in progress.-
dc.languageEnglish-
dc.publisherMUNKSGAARD INT PUBL LTD-
dc.subjectTUMOR-NECROSIS-FACTOR-
dc.subjectTNF RECEPTOR SUPERFAMILY-
dc.subjectINDUCED CELL-DEATH-
dc.subjectFACTOR-ALPHA-
dc.subjectCRYSTAL-STRUCTURE-
dc.subjectMUTATIONAL ANALYSIS-
dc.subjectINDUCED APOPTOSIS-
dc.subjectLIGAND-
dc.subjectFAMILY-
dc.subjectDOMAIN-
dc.titleExpression, purification and crystallization of recombinant human TRAIL-
dc.typeArticle-
dc.identifier.wosid000080399700030-
dc.identifier.scopusid2-s2.0-0033135957-
dc.type.rimsART-
dc.citation.volume55-
dc.citation.beginningpage1101-
dc.citation.endingpage1104-
dc.citation.publicationnameACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY-
dc.identifier.doi10.1107/S090744499900164X-
dc.contributor.localauthorOh, Byung-Ha-
dc.contributor.nonIdAuthorCha, SS-
dc.contributor.nonIdAuthorShin, HC-
dc.contributor.nonIdAuthorChoi, KY-
dc.type.journalArticleArticle-
dc.subject.keywordPlusTUMOR-NECROSIS-FACTOR-
dc.subject.keywordPlusTNF RECEPTOR SUPERFAMILY-
dc.subject.keywordPlusINDUCED CELL-DEATH-
dc.subject.keywordPlusFACTOR-ALPHA-
dc.subject.keywordPlusCRYSTAL-STRUCTURE-
dc.subject.keywordPlusMUTATIONAL ANALYSIS-
dc.subject.keywordPlusINDUCED APOPTOSIS-
dc.subject.keywordPlusLIGAND-
dc.subject.keywordPlusFAMILY-
dc.subject.keywordPlusDOMAIN-
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