Mitigating Clonal Variation in Recombinant Mammalian Cell Lines

Cited 0 time in webofscience Cited 0 time in scopus
  • Hit : 33
  • Download : 0
Mammalian expression platforms are primary production systems for therapeutic proteins that require complex post-translational modifications. Current processes used for developing recombinant mammalian cell lines generate clonal cell lines with high phenotypic heterogeneity, which has puzzled researchers that use mammalian cell culture systems for a long time. Advances in mammalian genome-editing technologies and systems biotechnology have shed light on clonal variation and enabled rational cell engineering in a targeted manner. We propose a new approach for a next-generation cell line development platform that can minimize clonal variation. Combined with the knowledge-based selection of ideal integration sites and engineering targets,targeted integration-based cell line development will allow tailored control of recombinant gene expression with predicted phenotypes.
Publisher
ELSEVIER SCIENCE LONDON
Issue Date
2019-09
Language
English
Article Type
Review
Citation

TRENDS IN BIOTECHNOLOGY, v.37, no.9, pp.931 - 942

ISSN
0167-7799
DOI
10.1016/j.tibtech.2019.02.007
URI
http://hdl.handle.net/10203/266086
Appears in Collection
BS-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0