Mitigating Clonal Variation in Recombinant Mammalian Cell Lines

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dc.contributor.authorLee, Jae Seongko
dc.contributor.authorKildegaard, Helene Faustrupko
dc.contributor.authorLewis, Nathan E.ko
dc.contributor.authorLee, Gyun Minko
dc.date.accessioned2019-09-03T01:20:05Z-
dc.date.available2019-09-03T01:20:05Z-
dc.date.created2019-06-10-
dc.date.issued2019-09-
dc.identifier.citationTRENDS IN BIOTECHNOLOGY, v.37, no.9, pp.931 - 942-
dc.identifier.issn0167-7799-
dc.identifier.urihttp://hdl.handle.net/10203/266086-
dc.description.abstractMammalian expression platforms are primary production systems for therapeutic proteins that require complex post-translational modifications. Current processes used for developing recombinant mammalian cell lines generate clonal cell lines with high phenotypic heterogeneity, which has puzzled researchers that use mammalian cell culture systems for a long time. Advances in mammalian genome-editing technologies and systems biotechnology have shed light on clonal variation and enabled rational cell engineering in a targeted manner. We propose a new approach for a next-generation cell line development platform that can minimize clonal variation. Combined with the knowledge-based selection of ideal integration sites and engineering targets,targeted integration-based cell line development will allow tailored control of recombinant gene expression with predicted phenotypes.-
dc.languageEnglish-
dc.publisherELSEVIER SCIENCE LONDON-
dc.titleMitigating Clonal Variation in Recombinant Mammalian Cell Lines-
dc.typeArticle-
dc.identifier.wosid000481640500006-
dc.identifier.scopusid2-s2.0-85063035419-
dc.type.rimsART-
dc.citation.volume37-
dc.citation.issue9-
dc.citation.beginningpage931-
dc.citation.endingpage942-
dc.citation.publicationnameTRENDS IN BIOTECHNOLOGY-
dc.identifier.doi10.1016/j.tibtech.2019.02.007-
dc.contributor.localauthorLee, Gyun Min-
dc.contributor.nonIdAuthorLee, Jae Seong-
dc.contributor.nonIdAuthorKildegaard, Helene Faustrup-
dc.contributor.nonIdAuthorLewis, Nathan E.-
dc.description.isOpenAccessN-
dc.type.journalArticleReview-
dc.subject.keywordPlusHAMSTER OVARY CELLS-
dc.subject.keywordPlusPROTEIN-PRODUCTION-
dc.subject.keywordPlusCHO-CELLS-
dc.subject.keywordPlusSYSTEMS BIOTECHNOLOGY-
dc.subject.keywordPlusGENE-EXPRESSION-
dc.subject.keywordPlusGENOME-
dc.subject.keywordPlusHETEROGENEITY-
dc.subject.keywordPlusINTEGRATION-
dc.subject.keywordPlusSTABILITY-
dc.subject.keywordPlusSEQUENCE-
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