Quantified High-Throughput Screening of Escherichia coli Producing Poly(3-hydroxybutyrate) Based on FACS

Cited 14 time in webofscience Cited 0 time in scopus
  • Hit : 31
  • Download : 0
Here, we report on a highly sensitive method for the detection of P(3HB) accumulation in Escherichia coli cells based on the automated flow cytometry system using fluorescent dyes. E. coli containing P(3HB) were stained with either BODIPY or Nile red fluorescent dye, and their staining properties were analyzed under a variety of conditions. Compared with Nile red, BODIPY was much more sensitive in staining P(3HB) and overall demonstrated a more rapid staining of cells, a greater resistance to photobleaching, and greater cell viability. In addition, we also successfully monitored heterogeneity in P(3HB) accumulation within a cell population using BODIPY staining and flow cytometry. We believe this optimized staining method using BODIPY in combination with screening by high-speed flow cytometer will be helpful in the engineering of host cells toward an enhanced production of bioplastics.
Publisher
HUMANA PRESS INC
Issue Date
2013-08
Language
English
Article Type
Article
Keywords

BETA-HYDROXYBUTYRIC ACID; PHA SYNTHASE; NILE RED; FLOW-CYTOMETRY; POLY-3-HYDROXYBUTYRATE; POLYHYDROXYALKANOATES; EVOLUTION; PROTEOME

Citation

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, v.170, no.7, pp.1767 - 1779

ISSN
0273-2289
DOI
10.1007/s12010-013-0311-2
URI
http://hdl.handle.net/10203/254753
Appears in Collection
CBE-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 14 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0