Quantitative phase imaging in biomedicine

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Quantitative phase imaging (QPI) has emerged as a valuable method for investigating cells and tissues. QPI operates on unlabelled specimens and, as such, is complementary to established fluorescence microscopy, exhibiting lower phototoxicity and no photobleaching. As the images represent quantitative maps of optical path length delays introduced by the specimen, QPI provides an objective measure of morphology and dynamics, free of variability due to contrast agents. Owing to the tremendous progress witnessed especially in the past 10-15 years, a number of technologies have become sufficiently reliable and translated to biomedical laboratories. Commercialization efforts are under way and, as a result, the QPI field is now transitioning from a technology-development-driven to an application-focused field. In this Review, we aim to provide a critical and objective overview of this dynamic research field by presenting the scientific context, main principles of operation and current biomedical applications.
Publisher
NATURE PUBLISHING GROUP
Issue Date
2018-10
Language
English
Article Type
Review
Citation

NATURE PHOTONICS, v.12, no.10, pp.578 - 589

ISSN
1749-4885
DOI
10.1038/s41566-018-0253-x
URI
http://hdl.handle.net/10203/247641
Appears in Collection
PH-Journal Papers(저널논문)
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