Super-resolution three-dimensional fluorescence and optical diffraction tomography of live cells using structured illumination generated by a digital micromirror device

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We present a multimodal approach for measuring the three-dimensional (3D) refractive index (RI) and fluorescence distributions of live cells by combining optical diffraction tomography (ODT) and 3D structured illumination microscopy (SIM). A digital micromirror device is utilized to generate structured illumination patterns for both ODT and SIM, which enables fast and stable measurements. To verify its feasibility and applicability, the proposed method is used to measure the 3D RI distribution and 3D fluorescence image of various samples, including a cluster of fluorescent beads, and the time-lapse 3D RI dynamics of fluorescent beads inside a HeLa cell, from which the trajectory of the beads in the HeLa cell is analyzed using spatiotemporal correlations.
Publisher
NATURE PUBLISHING GROUP
Issue Date
2018-06
Language
English
Article Type
Article
Citation

SCIENTIFIC REPORTS, v.8, pp.9183

ISSN
2045-2322
DOI
10.1038/s41598-018-27399-w
URI
http://hdl.handle.net/10203/244041
Appears in Collection
PH-Journal Papers(저널논문)
Files in This Item
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