Caenorhabditus elegans arrestin regulates neural G protein signaling and olfactory adaptation and recovery

Cited 40 time in webofscience Cited 0 time in scopus
  • Hit : 264
  • Download : 0
Although regulation of G protein-coupled receptor signaling by receptor kinases and arrestins is a well established biochemical process, the physiological significance of such regulation remains poorly understood. To better understand the in vivo consequences of arrestin function, we have examined the function of the sole arrestin in Caenorhabditis elegans (ARR-1). ARR-1 is primarily expressed in the nervous system, including the HSN neuron and various chemosensory neurons involved in detecting soluble and volatile odorants. arr-1 null mutants exhibit normal chemotaxis but have significant defects in olfactory adaptation and recovery to volatile odorants. In contrast, adaptation is enhanced in animals overexpressing ARR-1. Both the adaptation and recovery defects of arr-1 mutants are rescued by transgenic expression of wild-type ARR-1, whereas expression of a C-terminally truncated ARR-1 effectively rescues only the adaptation defect. A potential mechanistic basis for these findings is revealed by in vitro studies demonstrating that wild-type ARR-1 binds proteins of the endocytic machinery and promotes receptor endocytosis, whereas C-terminally truncated ARR-1 does not. These results demonstrate that ARR-1 functions to regulate chemosensory signaling, enabling organisms to adapt to a variety of environmental cues, and provide an in vivo link between arrestin, receptor endocytosis, and temporal recovery from adaptation.
Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
Issue Date
2005-07
Language
English
Article Type
Article
Keywords

COUPLED RECEPTOR TRAFFICKING; C-ELEGANS; BETA-ARRESTIN; BETA(2)-ADRENERGIC RECEPTOR; CRYSTAL-STRUCTURE; CLATHRIN; ENDOCYTOSIS; ADAPTER; GENE; ACTIVATION

Citation

JOURNAL OF BIOLOGICAL CHEMISTRY, v.280, no.26, pp.24649 - 24662

ISSN
0021-9258
DOI
10.1074/jbc.M502637200
URI
http://hdl.handle.net/10203/240864
Appears in Collection
MSE-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 40 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0