The Drosha-DGCR8 complex in primary microRNA processing

Cited 1151 time in webofscience Cited 0 time in scopus
  • Hit : 135
  • Download : 0
RNase III proteins play key roles in microRNA (miRNA) biogenesis. The nuclear RNase III Drosha cleaves primary miRNAs (pri-miRNAs) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic RNase III Dicer to generate mature miRNAs. While Dicer (class III) and other simple RNase III proteins (class I) have been studied intensively, the class II enzyme Drosha remains to be characterized. Here we dissected the action mechanism of human Drosha by generating mutants and by characterizing its new interacting partner, DGCR8. The basic action mechanism of Drosha was found to be similar to that of human Dicer; the RNase III domains A and B form an intramolecular dimer and cleave the 3' and 5' strands of the stem, respectively. Human Drosha fractionates at similar to650 kDa, indicating that Drosha functions as a large complex. In this complex, Drosha interacts with DGCR8, which contains two double-stranded RNA (dsRNA)-binding domains. By RNAi and biochemical reconstitution, we show that DGCR8 may be an essential component of the pri-miRNA processing complex, along with Drosha. Based on these results, we propose a model for the action mechanism of class II RNase III proteins.
Publisher
COLD SPRING HARBOR LAB PRESS
Issue Date
2004-12
Language
English
Article Type
Article
Keywords

DSRNA-BINDING-PROTEIN; RNA-INTERFERENCE; NUCLEAR EXPORT; HUMAN DICER; C-ELEGANS; PAZ DOMAIN; PRECURSORS; RIBONUCLEASE; BIOGENESIS; ARGONAUTE2

Citation

GENES & DEVELOPMENT, v.18, no.24, pp.3016 - 3027

ISSN
0890-9369
DOI
10.1101/gad.1262504
URI
http://hdl.handle.net/10203/221054
Appears in Collection
MSE-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 1151 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0