Characterization of DGCR8/Pasha, the essential cofactor for Drosha in primary miRNA processing

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DGCR8/Pasha is an essential cofactor for Drosha, a nuclear RNase III that cleaves the local hairpin structures embedded in long primary microRNA transcripts (pri-miRNAs) in eukaryotes. Although our knowledge of pri-miRNA processing has significantly advanced in recent years, the precise role of DGCR8 in this pathway remains unclear. In our present study, we dissect the domains in DGCR8 that contribute to the processing of pri-miRNAs and the subcellular localization of DGCR8. Drosha is stabilized through an interaction between its middle domain and the conserved C-terminal domain of DGCR8. Furthermore, DGCR8, but not Drosha, can directly and stably interact with pri-miRNAs, and the tandem dsRNA-binding domains (dsRBDs) in DGCR8 are responsible for this recognition. Moreover, the DGCR8 N-terminal region upstream of its dsRBDs is unnecessary for pri-miRNA processing but is critical for nuclear localization. Our study thus provides further insights into the mechanism of action of the Drosha-DGCR8 complex in pri-miRNA processing.
Publisher
OXFORD UNIV PRESS
Issue Date
2006-09
Language
English
Article Type
Article
Keywords

DSRNA-BINDING-PROTEIN; RNA-INTERFERENCE; MICRORNA MATURATION; NUCLEAR EXPORT; C-ELEGANS; DROSOPHILA-MELANOGASTER; MICROPROCESSOR COMPLEX; BIOGENESIS; EXPRESSION; GENES

Citation

NUCLEIC ACIDS RESEARCH, v.34, no.16, pp.4622 - 4629

ISSN
0305-1048
DOI
10.1093/nar/gkl458
URI
http://hdl.handle.net/10203/221051
Appears in Collection
MSE-Journal Papers(저널논문)
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