Generation of PDGFR alpha(+) Cardioblasts from Pluripotent Stem Cells

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Isolating actively proliferating cardioblasts is the first crucial step for cardiac regeneration through cell implantation. However, the origin and identity of putative cardioblasts are still unclear. Here, we uncover a novel class of cardiac lineage cells, PDGFR alpha(+) Flk1(-) cardioblasts (PCBs), from mouse and human pluripotent stem cells induced using CsAYTE, a combination of the small molecules Cyclosporin A, the rho-associated coiled-coil kinase inhibitor Y27632, the antioxidant Trolox, and the ALK5 inhibitor EW7197. This novel population of actively proliferating cells is cardiac lineage-committed but in a morphologically and functionally immature state compared to mature cardiomyocytes. Most important, most of CsAYTE-induced PCBs spontaneously differentiated into functional alpha MHC+ cardiomyocytes (M+ CMs) and could be a potential cellular resource for cardiac regeneration.
Publisher
NATURE PUBLISHING GROUP
Issue Date
2017-02
Language
English
Article Type
Article
Keywords

CARDIOVASCULAR PROGENITOR CELLS; CARDIAC PROGENITORS; PRECURSOR CELLS; SMOOTH-MUSCLE; DIFFERENTIATION; CARDIOMYOCYTE; LINEAGE; MULTIPOTENT; MOUSE; HEART

Citation

SCIENTIFIC REPORTS, v.7

ISSN
2045-2322
DOI
10.1038/srep41840
URI
http://hdl.handle.net/10203/220869
Appears in Collection
MSE-Journal Papers(저널논문)BS-Journal Papers(저널논문)
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