High cell density culture of metabolically engineered Escherichia coli for the production of poly(3-hydroxybutyrate) in a defined medium

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A recombinant Escherichia coil strain XL1-Blue harboring a stable high-copy-number plasmid pSYL107 containing the Alcaligenes eutrophus polyhydroxyalkanoate biosynthesis genes and the Escherichia coil ftsZ gene was employed for the production of poly(3-hydroxybutyrate) (PHB) by fed-batch culture in a defined medium. Suppression of filamentation by overexpressing the cell division protein FtsZ allowed production of PHB to a high concentration (77 g/L) with high productivity (2 g/L/h) in a defined medium, which was not possible with the recombinant E. coli that underwent filamentation. Further optimization of fed-batch culture condition resulted in PHB concentration of 104 g/L in a defined medium, which was the highest value reported to date by employing recombinant E. coli. (C) 1998 John Wiley & Sons, Inc.
Publisher
JOHN WILEY SONS INC
Issue Date
1998-04
Language
English
Article Type
Article
Citation

BIOTECHNOLOGY AND BIOENGINEERING, v.58, no.2-3, pp.325 - 328

ISSN
0006-3592
URI
http://hdl.handle.net/10203/20532
Appears in Collection
CBE-Journal Papers(저널논문)
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