DC Field | Value | Language |
---|---|---|
dc.contributor.author | Wang, FL | ko |
dc.contributor.author | Lee, SangYup | ko |
dc.date.accessioned | 2010-11-30T06:57:49Z | - |
dc.date.available | 2010-11-30T06:57:49Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1998-04 | - |
dc.identifier.citation | BIOTECHNOLOGY AND BIOENGINEERING, v.58, no.2-3, pp.325 - 328 | - |
dc.identifier.issn | 0006-3592 | - |
dc.identifier.uri | http://hdl.handle.net/10203/20532 | - |
dc.description.abstract | A recombinant Escherichia coil strain XL1-Blue harboring a stable high-copy-number plasmid pSYL107 containing the Alcaligenes eutrophus polyhydroxyalkanoate biosynthesis genes and the Escherichia coil ftsZ gene was employed for the production of poly(3-hydroxybutyrate) (PHB) by fed-batch culture in a defined medium. Suppression of filamentation by overexpressing the cell division protein FtsZ allowed production of PHB to a high concentration (77 g/L) with high productivity (2 g/L/h) in a defined medium, which was not possible with the recombinant E. coli that underwent filamentation. Further optimization of fed-batch culture condition resulted in PHB concentration of 104 g/L in a defined medium, which was the highest value reported to date by employing recombinant E. coli. (C) 1998 John Wiley & Sons, Inc. | - |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | JOHN WILEY SONS INC | - |
dc.title | High cell density culture of metabolically engineered Escherichia coli for the production of poly(3-hydroxybutyrate) in a defined medium | - |
dc.type | Article | - |
dc.identifier.wosid | 000072158500033 | - |
dc.identifier.scopusid | 2-s2.0-2642650308 | - |
dc.type.rims | ART | - |
dc.citation.volume | 58 | - |
dc.citation.issue | 2-3 | - |
dc.citation.beginningpage | 325 | - |
dc.citation.endingpage | 328 | - |
dc.citation.publicationname | BIOTECHNOLOGY AND BIOENGINEERING | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Lee, SangYup | - |
dc.contributor.nonIdAuthor | Wang, FL | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | poly(3-hydroxybutyrate) | - |
dc.subject.keywordAuthor | Escherichia coli | - |
dc.subject.keywordAuthor | filamentation suppression | - |
dc.subject.keywordAuthor | defined medium | - |
dc.subject.keywordAuthor | high cell density culture | - |
dc.subject.keywordPlus | BACTERIAL POLYHYDROXYALKANOATES | - |
dc.subject.keywordPlus | ACID) | - |
dc.subject.keywordPlus | STRAINS | - |
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