Water-free microencapsulation of proteins within PLGA microparticles by spray drying using PEG-assisted protein solubilization technique in organic solvent

Poly(D,L-lactic-co-glycolic acid) (PLGA) microparticles encapsulating therapeutic proteins were prepared under a water-free formulation condition. Bovine serum albumin (BSA) and recombinant human growth hormone (rhGH) were homogeneously solubilized as nano-scale complexes in methylene chloride phase by using polyethylene glycol (PEG) as a complex-forming agent. The organic phase containing dissolved PLGA and PEG/protein complexes was directly spray dried to obtain PLGA microparticles encapsulating proteins. They exhibited sustained release profiles of BSA and rhGH up to 30 days with reduced initial bursts. The released protein molecules from the microparticles maintained structural integrity without aggregation, suggesting that the current single-step protein microencapsulation method without using water could be potentially applied for sustained delivery of a wide range of therapeutic protein drugs that are not soluble in organic solvents. (c) 2008 Elsevier B.V. All rights reserved.
Publisher
ELSEVIER SCIENCE BV
Issue Date
2008-09
Language
ENG
Keywords

HUMAN GROWTH-HORMONE; BIODEGRADABLE MICROSPHERES; SUSTAINED-RELEASE; POLY(ETHYLENE GLYCOL); ACID) MICROSPHERES; GENE DELIVERY; ENCAPSULATION; STABILITY; PRECIPITATION; DEGRADATION

Citation

EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, v.70, no.1, pp.137 - 144

ISSN
0939-6411
DOI
10.1016/j.ejpb.2008.04.006
URI
http://hdl.handle.net/10203/12395
Appears in Collection
BS-Journal Papers(저널논문)
  • Hit : 158
  • Download : 22
  • Cited 0 times in thomson ci
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡClick to seewebofscience_button
⊙ Cited 24 items in WoSClick to see citing articles inrecords_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0