We report on the fabrication of well-aligned multi-segment line patterns over large areas featuring dimensional and compositional exquisite tunability using a combination of photolithography and soft-lithography techniques. We show that thus this new top-down approach has great advantages and that it is beneficial by increasing the control of the multi-segment line width and pattern feature dimensions ranging from microns to a few hundred nanometres. Various combinations of multisegment materials with full control over the periodic alignment, which include Au-Ni, Au-Cu and Au-Ag, were prepared by simply changing the metals evaporated before the lift-off process. Au-Ni multi-segment metal line patterns showed a linear current-voltage response, identical with that of a line pattern from a single material. Thus, one can take advantage of the simple electrical properties of the 1-dimensional nanostructure. Our approach provides great potential in practical fabrication of well-integrated multi-metal component devices for electrical and optical detection.