Sample stacking capillary electrophoretic microdevice for highly sensitive mini Y short tandem repeat genotyping

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dc.contributor.authorChen, Yuchaoko
dc.contributor.authorChoi, Jong-Youngko
dc.contributor.authorChoi, Seok-Jinko
dc.contributor.authorSeo, Tae-Seokko
dc.date.accessioned2013-03-11T15:38:00Z-
dc.date.available2013-03-11T15:38:00Z-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.issued2010-09-
dc.identifier.citationELECTROPHORESIS, v.31, no.17, pp.2974 - 2980-
dc.identifier.issn0173-0835-
dc.identifier.urihttp://hdl.handle.net/10203/99491-
dc.description.abstractLab-on-a-chip provides an ideal platform for short tandem repeat (STR) genotyping due to its intrinsic low sample consumption, rapid analysis, and high-throughput capability. One of the challenges, however, in the forensic human identification on the microdevice is the detection sensitivity derived from the nanoliter volume sample handling. To overcome such a sensitivity issue, here we developed a sample stacking CE microdevice for mini Y STR genotyping. The mini Y STR includes redesigned primer sequences to generate smaller-sized PCR amplicons to enhance the PCR efficiency and the success rate for a low copy number and degraded DNA. The mini Y STR amplicons occupied in the 5- and 10-mm stacking microchannels are preconcentrated efficiently in a defined narrow region through the optimized sample stacking CE scheme, resulting in more than tenfold improved fluorescence peak intensities compared with that of a conventional cross-injection microcapillary electrophoresis method. Such signal enhancement allows us to successfully analyze the Y STR typing with only 25 pg of male genomic DNA, with high background of female genomic DNA, and with highly degraded male genomic DNA. The combination of the mini Y STR system with the novel sample stacking CE microdevice provides the highly sensitive Y STR typing on a chip, making it promising to perform high-performance on-site forensic human identification.-
dc.languageEnglish-
dc.publisherWILEY-V C H VERLAG GMBH-
dc.subjectTRANSFER-LABELED PRIMERS-
dc.subjectSOLID-PHASE EXTRACTION-
dc.subjectGENETIC-ANALYSIS-
dc.subjectDNA-
dc.subjectMICROCHIP-
dc.subjectSYSTEM-
dc.subjectPURIFICATION-
dc.subjectPROTEINS-
dc.titleSample stacking capillary electrophoretic microdevice for highly sensitive mini Y short tandem repeat genotyping-
dc.typeArticle-
dc.identifier.wosid000282241500014-
dc.identifier.scopusid2-s2.0-77956675222-
dc.type.rimsART-
dc.citation.volume31-
dc.citation.issue17-
dc.citation.beginningpage2974-
dc.citation.endingpage2980-
dc.citation.publicationnameELECTROPHORESIS-
dc.identifier.doi10.1002/elps.201000270-
dc.embargo.liftdate9999-12-31-
dc.embargo.terms9999-12-31-
dc.contributor.localauthorSeo, Tae-Seok-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorCE-
dc.subject.keywordAuthorDetection sensitivity-
dc.subject.keywordAuthorMini Y STR-
dc.subject.keywordAuthorSample stacking-
dc.subject.keywordPlusTRANSFER-LABELED PRIMERS-
dc.subject.keywordPlusSOLID-PHASE EXTRACTION-
dc.subject.keywordPlusGENETIC-ANALYSIS-
dc.subject.keywordPlusDNA-
dc.subject.keywordPlusMICROCHIP-
dc.subject.keywordPlusSYSTEM-
dc.subject.keywordPlusPURIFICATION-
dc.subject.keywordPlusPROTEINS-
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