DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Se Jin | ko |
dc.contributor.author | Choi, Seok Jin | ko |
dc.contributor.author | Neelamegam, Rameshkumar | ko |
dc.contributor.author | SEO, TAE SEOK | ko |
dc.date.accessioned | 2013-03-11T13:04:19Z | - |
dc.date.available | 2013-03-11T13:04:19Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2010-03 | - |
dc.identifier.citation | BIOCHIP JOURNAL, v.4, no.1, pp.42 - 48 | - |
dc.identifier.issn | 1976-0280 | - |
dc.identifier.uri | http://hdl.handle.net/10203/99384 | - |
dc.description.abstract | Here we present telomerase activity screening using a capillary electrophoretic (CE) microdevice for human cancer diagnostics. The telomerase enzyme, a contributor to the maintenance of telomere length in cancer cells, was extracted from various human cells including MCF-7 (Human breast cancer cells), A549 (Human lung cancer cells), and SK-N-SH (Human neuroblastoma cells), and then telomeric repeat amplification protocol (TRAP)-based genetic analysis was performed to evaluate the activity of telomerase enzyme. The resultant 6-bp tandem repeat PCR amplicons derived from cancer cells were separated and detected on the 6 cm-long CE microdevice within 5 min. A limit of detection of 5 cells was achieved to analyze the telomerase activity on a CE microchip. In comparison with a conventional PAGE method, micro-fabricated CE-based analysis has many advantages in terms of high speed, low sample consumption, and high sensitive detection. The successful demonstration of telomerase activity screening on a chip implies great potential of a complete integration of a sample preparation and PCR unit in a single format for high-performance cancer diagnostics in a clinical arena. | - |
dc.language | English | - |
dc.publisher | SPRINGER | - |
dc.subject | POLYMERASE-CHAIN-REACTION | - |
dc.subject | ENERGY-TRANSFER | - |
dc.subject | IMMORTAL CELLS | - |
dc.subject | ASSAY | - |
dc.subject | AMPLIFICATION | - |
dc.subject | HYBRIDIZATION | - |
dc.subject | INJECTION | - |
dc.subject | PRIMERS | - |
dc.subject | ENZYME | - |
dc.subject | GEL | - |
dc.title | Microchip-based capillary electrophoretic analysis of telomerase activity for cancer diagnostics | - |
dc.type | Article | - |
dc.identifier.wosid | 000275902400007 | - |
dc.identifier.scopusid | 2-s2.0-77956693271 | - |
dc.type.rims | ART | - |
dc.citation.volume | 4 | - |
dc.citation.issue | 1 | - |
dc.citation.beginningpage | 42 | - |
dc.citation.endingpage | 48 | - |
dc.citation.publicationname | BIOCHIP JOURNAL | - |
dc.identifier.doi | 10.1007/s13206-010-4107-y | - |
dc.contributor.localauthor | SEO, TAE SEOK | - |
dc.contributor.nonIdAuthor | Neelamegam, Rameshkumar | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Telomerase | - |
dc.subject.keywordAuthor | Telomeric repeat amplification | - |
dc.subject.keywordAuthor | Polymerase chain reaction | - |
dc.subject.keywordAuthor | Capillary electrophoretic microdevice | - |
dc.subject.keywordAuthor | Cancer diagnostics | - |
dc.subject.keywordPlus | POLYMERASE-CHAIN-REACTION | - |
dc.subject.keywordPlus | ENERGY-TRANSFER | - |
dc.subject.keywordPlus | IMMORTAL CELLS | - |
dc.subject.keywordPlus | ASSAY | - |
dc.subject.keywordPlus | AMPLIFICATION | - |
dc.subject.keywordPlus | HYBRIDIZATION | - |
dc.subject.keywordPlus | INJECTION | - |
dc.subject.keywordPlus | PRIMERS | - |
dc.subject.keywordPlus | ENZYME | - |
dc.subject.keywordPlus | GEL | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.