DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chang, Ho-Nam | ko |
dc.contributor.author | Kim, Nag-Jong | ko |
dc.contributor.author | Kang, Jong-Won | ko |
dc.contributor.author | Jeong, Chang-Moon | ko |
dc.contributor.author | Choi, Jin-Dal-Rae | ko |
dc.contributor.author | Fei, Qiang | ko |
dc.contributor.author | Kim, Byoung-Jin | ko |
dc.contributor.author | Kwon, Sun-Hoon | ko |
dc.contributor.author | Lee, Sang-Yup | ko |
dc.contributor.author | Kim, Jung-Bae | ko |
dc.date.accessioned | 2013-03-11T04:12:32Z | - |
dc.date.available | 2013-03-11T04:12:32Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2011-05 | - |
dc.identifier.citation | BIOPROCESS AND BIOSYSTEMS ENGINEERING, v.34, no.4, pp.419 - 431 | - |
dc.identifier.issn | 1615-7591 | - |
dc.identifier.uri | http://hdl.handle.net/10203/98221 | - |
dc.description.abstract | We carried out the first simulation on multi-stage continuous high cell density culture (MSC-HCDC) to show that the MSC-HCDC can achieve batch/fed-batch product titer with much higher productivity to the fed-batch productivity using published fermentation kinetics of lactic acid, penicillin and ethanol. The system under consideration consists of n-serially connected continuous stirred-tank reactors (CSTRs) with either hollow fiber cell recycling or cell immobilization for high cell-density culture. In each CSTR substrate supply and product removal are possible. Penicillin production is severely limited by glucose metabolite repression that requires multi-CSTR glucose feeding. An 8-stage C-HCDC lactic acid fermentation resulted in 212.9 g/L of titer and 10.6 g/L/h of productivity, corresponding to 101 and 429% of the comparable lactic acid fed-batch, respectively. The penicillin production model predicted 149% (0.085 g/L/h) of productivity in 8-stage C-HCDC with 40 g/L of cell density and 289% of productivity (0.165 g/L/h) in 7-stage C-HCDC with 60 g/L of cell density compared with referring batch cultivations. A 2-stage C-HCDC ethanol experimental run showed 107% titer and 257% productivity of the batch system having 88.8 g/L of titer and 3.7 g/L/h of productivity. MSC-HCDC can give much higher productivity than batch/fed-batch system, and yield a several percentage higher titer as well. The productivity ratio of MSC-HCDC over batch/fed-batch system is given as a multiplication of system dilution rate of MSC-HCDC and cycle time of batch/fed-batch system. We suggest MSC-HCDC as a new production platform for various fermentation products including monoclonal antibody. | - |
dc.language | English | - |
dc.publisher | SPRINGER | - |
dc.title | Multi-stage high cell continuous fermentation for high productivity and titer | - |
dc.type | Article | - |
dc.identifier.wosid | 000289434700004 | - |
dc.identifier.scopusid | 2-s2.0-79956208508 | - |
dc.type.rims | ART | - |
dc.citation.volume | 34 | - |
dc.citation.issue | 4 | - |
dc.citation.beginningpage | 419 | - |
dc.citation.endingpage | 431 | - |
dc.citation.publicationname | BIOPROCESS AND BIOSYSTEMS ENGINEERING | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Chang, Ho-Nam | - |
dc.contributor.localauthor | Lee, Sang-Yup | - |
dc.contributor.nonIdAuthor | Kim, Jung-Bae | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Multi-stage continuous fermentation | - |
dc.subject.keywordAuthor | High cell density | - |
dc.subject.keywordAuthor | Ethanol | - |
dc.subject.keywordAuthor | Lactic acid | - |
dc.subject.keywordAuthor | Antibody | - |
dc.subject.keywordPlus | CONTINUOUS ETHANOL-PRODUCTION | - |
dc.subject.keywordPlus | RECOMBINANT ANTIBODY-PRODUCTION | - |
dc.subject.keywordPlus | LACTIC-ACID FERMENTATION | - |
dc.subject.keywordPlus | HOLLOW-FIBER BIOREACTOR | - |
dc.subject.keywordPlus | IMMOBILIZED YEAST-CELLS | - |
dc.subject.keywordPlus | FILTER PERFUSION SYSTEM | - |
dc.subject.keywordPlus | RECYCLE BIOREACTOR | - |
dc.subject.keywordPlus | SACCHAROMYCES-CEREVISIAE | - |
dc.subject.keywordPlus | DIRECTED EVOLUTION | - |
dc.subject.keywordPlus | CULTURE | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.