Determination of the Intracellular Concentrations of Metabolites in Escherichia coli Collected during the Exponential and Stationary Growth Phases using Liquid Chromatography-Mass Spectrometry

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In the present study, we demonstrate that SRM LC-MS/MS method developed by Luo et al. (ref. 10) can be successfully applied to the quantitative analysis of intracellular metabolites in E. coli that are collected at the exponential and stationary growth phases. A focus is given on measuring the changes in the concentrations of intracellular metabolites in batch cultures, which were induced during both the dynamically changing exponential and stationary growth phases. The following intracellular metabolites are quantified in the exponential and stationary phases of E. coli growth, using the SRM mode of a triple quadrupole mass spectrometer: glucose-1-phosphate, fructose-1,6-bisphosphate, phosphoenolpyruvate, pyruvate, acetyl-coenzyme A, 6-phosphogluconate, ribulose-5-phosphate, xylulose-5-phosphate, erythrose-4-phosphate. The determined intracellular metabolite concentration profiles are shown to be in a good agreement with the growth profiles of E. coli, which clearly indicates that SRM LC-MS/MS can be successfully used for following the metabolite changes induced at different growth stages.
Publisher
KOREAN CHEMICAL SOC
Issue Date
2011-02
Language
English
Article Type
Article
Keywords

PENTOSE-PHOSPHATE PATHWAY; ELECTROSPRAY-IONIZATION; METABOLOMICS; TECHNOLOGIES; EXTRACTION; GENOMICS

Citation

BULLETIN OF THE KOREAN CHEMICAL SOCIETY, v.32, no.2, pp.524 - 530

ISSN
0253-2964
URI
http://hdl.handle.net/10203/98119
Appears in Collection
CBE-Journal Papers(저널논문)
Files in This Item
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