DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Jin Hwan | ko |
dc.contributor.author | Jang, Yu-Sin | ko |
dc.contributor.author | Lee, Jeong Wook | ko |
dc.contributor.author | Lee, SangYup | ko |
dc.date.accessioned | 2013-03-11T03:16:07Z | - |
dc.date.available | 2013-03-11T03:16:07Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2011-05 | - |
dc.identifier.citation | BIOTECHNOLOGY AND BIOENGINEERING, v.108, no.5, pp.1140 - 1147 | - |
dc.identifier.issn | 0006-3592 | - |
dc.identifier.uri | http://hdl.handle.net/10203/98116 | - |
dc.description.abstract | A less frequently employed Escherichia coli strain W, yet possessing useful metabolic characteristics such as less acetic acid production and high L-valine tolerance, was metabolically engineered for the production of L-valine. The ilvA gene was deleted to make more pyruvate, a key precursor for L-valine, available for enhanced L-valine biosynthesis. The lacI gene was deleted to allow constitutive expression of genes under the tac or trc promoter. The ilvBN(mut) genes encoding feedback-resistant acetohydroxy acid synthase (AHAS) I and the L-valine biosynthetic ilvCED genes encoding acetohydroxy acid isomeroreductase, dihydroxy acid dehydratase, and branched chain amino acid aminotransferase, respectively, were amplified by plasmid-based overexpression. The global regulator Lrp and L-valine exporter YgaZH were also amplified by plasmid-based overexpression. The engineered E. coli W (Delta lacI Delta ilvA) strain overexpressing the ilvBN(mut), ilvCED, ygaZH, and lrp genes was able to produce an impressively high concentration of 60.7 g/L L-valine by fed-batch culture in 29.5 h, resulting in a high volumetric productivity of 2.06 g/L/h. The most notable finding is that there was no other byproduct produced during L-valine production. The results obtained in this study suggest that E. coli W can be a good alternative to Corynebacterium glutamicum and E. coli K-12, which have so far been the most efficient L-valine producer. Furthermore, it is expected that various bioproducts including other amino acids might be more efficiently produced by this revisited platform strain of E. coli. Biotechnol. Bioeng. 2011;108: 1140-1147. (C) 2010 Wiley Periodicals, Inc. | - |
dc.language | English | - |
dc.publisher | WILEY-BLACKWELL | - |
dc.title | Escherichia coli W as a New Platform Strain for the Enhanced Production of L-Valine by Systems Metabolic Engineering | - |
dc.type | Article | - |
dc.identifier.wosid | 000288394300016 | - |
dc.identifier.scopusid | 2-s2.0-79953162662 | - |
dc.type.rims | ART | - |
dc.citation.volume | 108 | - |
dc.citation.issue | 5 | - |
dc.citation.beginningpage | 1140 | - |
dc.citation.endingpage | 1147 | - |
dc.citation.publicationname | BIOTECHNOLOGY AND BIOENGINEERING | - |
dc.identifier.doi | 10.1002/bit.23044 | - |
dc.contributor.localauthor | Lee, SangYup | - |
dc.contributor.nonIdAuthor | Jang, Yu-Sin | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | L-valine | - |
dc.subject.keywordAuthor | L-valine tolerance | - |
dc.subject.keywordAuthor | Escherichia coli W strain | - |
dc.subject.keywordAuthor | metabolic engineering | - |
dc.subject.keywordPlus | CORYNEBACTERIUM-GLUTAMICUM | - |
dc.subject.keywordPlus | CARBON SOURCE | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | GENES | - |
dc.subject.keywordPlus | K-12 | - |
dc.subject.keywordPlus | LRP | - |
dc.subject.keywordPlus | TRANSCRIPTION | - |
dc.subject.keywordPlus | RESISTANCE | - |
dc.subject.keywordPlus | BACTERIA | - |
dc.subject.keywordPlus | LEUCINE | - |
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