DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kwon, YD | ko |
dc.contributor.author | Kim, S | ko |
dc.contributor.author | Lee, SangYup | ko |
dc.contributor.author | Kim, P | ko |
dc.date.accessioned | 2013-03-11T03:11:36Z | - |
dc.date.available | 2013-03-11T03:11:36Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2011-01 | - |
dc.identifier.citation | JOURNAL OF BIOSCIENCE AND BIOENGINEERING, v.111, no.1, pp.26 - 30 | - |
dc.identifier.issn | 1389-1723 | - |
dc.identifier.uri | http://hdl.handle.net/10203/98107 | - |
dc.description.abstract | To understand the responses of Escherichia coil to high succinate stress and to determine the roles of upregulated genes in high succinate tolerance, a continuous culture of wild-type E. coil W3110 was performed for 268 days in a gradually increasing concentration of succinate. Growth of the final adapted strain, designated DST160, proceeded growth rate of 0.20 h(-1) without a lag phase in medium containing 0.592 M succinate, while the wild-type strain showed 0.02 h(-1) in 38 h. The growth rates of DST160 in media containing either 0.61 M NaCl, 0.61 M KCl, or at pH 4.5 were 25% higher, 18% lower, and 57% higher than those of wild-type, respectively, implying DST160 acquired salt tolerance and pH shock tolerance as well as succinate tolerance. DNA microarray and real-time PCR results indicated that genes controlling active transport and biosynthesis of osmoprotectants were upregulated in DST160 compared to W3110. When ygjE, encoding a putative tartrate/succinate antiporter, and betA, encoding betaine biosynthesis, were expressed in a wild-type E. con as represent genes for active transport and osmoprotectant synthesis, respectively, greater growth rates were achieved under 0.592 M succinate stress conditions (seven times higher due to ygjE expression and six times higher due to betA expression) than wild-type. The potential to design a metabolic engineering for microbial succinate production is suggested based on the transcriptional regulation of the long-term adapted DST160. (C) 2010, The Society for Biotechnology, Japan. All rights reserved. | - |
dc.language | English | - |
dc.publisher | SOC BIOSCIENCE BIOENGINEERING JAPAN | - |
dc.title | Long-term continuous adaptation of Escherichia coli to high succinate stress and transcriptome analysis of the tolerant strain | - |
dc.type | Article | - |
dc.identifier.wosid | 000287427200007 | - |
dc.identifier.scopusid | 2-s2.0-78650987469 | - |
dc.type.rims | ART | - |
dc.citation.volume | 111 | - |
dc.citation.issue | 1 | - |
dc.citation.beginningpage | 26 | - |
dc.citation.endingpage | 30 | - |
dc.citation.publicationname | JOURNAL OF BIOSCIENCE AND BIOENGINEERING | - |
dc.identifier.doi | 10.1016/j.jbiosc.2010.08.007 | - |
dc.contributor.localauthor | Lee, SangYup | - |
dc.contributor.nonIdAuthor | Kwon, YD | - |
dc.contributor.nonIdAuthor | Kim, S | - |
dc.contributor.nonIdAuthor | Kim, P | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | High succinate stress | - |
dc.subject.keywordAuthor | Continuous adaptation | - |
dc.subject.keywordAuthor | Transcriptome | - |
dc.subject.keywordAuthor | ygjE | - |
dc.subject.keywordAuthor | betA | - |
dc.subject.keywordPlus | GLYCINE BETAINE | - |
dc.subject.keywordPlus | MANNHEIMIA-SUCCINICIPRODUCENS | - |
dc.subject.keywordPlus | ENHANCED PRODUCTION | - |
dc.subject.keywordPlus | ACID PRODUCTION | - |
dc.subject.keywordPlus | OSMOTIC-STRESS | - |
dc.subject.keywordPlus | TEMPERATURE | - |
dc.subject.keywordPlus | METABOLISM | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | RESPONSES | - |
dc.subject.keywordPlus | GENES | - |
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