Gene-expression Analysis of Acidic pH Shock Effects on Two-component Systems in Streptomyces coelicolor

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In this study, transcriptional analyses by using reverse transcription-polymerase chain reaction (RT-PCR), DNA chip, and quantitative real time-PCR were performed to investigate effects of acidic pH shock on two-component systems in Streptomyces coelicolor A3(2). Two-component systems of cseC/cseB and vanS/vanR, known to be closely linked with self-protection against cell wall hydrolysis caused by external stimuli were upregulated by the pH shock. The chiS/chiR, afsQ2/afsQ1, ecrA2/ecrA1, bldM, ramC/ramR, and ragK/ragR known to be positively associated with the initiation of secondary metabolism were also upregulated. The cutS/cutR known to be negatively related to the secondary metabolism was, however, slightly downregulated. Upregulation or downregulation by the acidic pH shock of these two-component regulator systems might have contributed in a concerted manner to the enhancement of secondary metabolite production, at least, in this particular case of actinorhodin production in S. coelicolor A3(2). (C) KSBB
Publisher
KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
Issue Date
2009-09
Language
English
Article Type
Article
Keywords

SIGNAL-TRANSDUCTION SYSTEM; SECONDARY METABOLISM; MORPHOLOGICAL-DIFFERENTIATION; KASUGAMYCIN PRODUCTION; REGULATORY SYSTEM; A3(2); ACTINORHODIN; VANCOMYCIN; RESISTANCE; ANTIBIOTICS

Citation

BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.14, no.5, pp.584 - 590

ISSN
1226-8372
DOI
10.1007/s12257-008-0260-8
URI
http://hdl.handle.net/10203/95028
Appears in Collection
CBE-Journal Papers(저널논문)
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