Identification and Characterization of a Xyloglucan-Specific Family 74 Glycosyl Hydrolase from Streptomyces coelicolor A3(2)

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The sco6545 gene of Streptomyces coelicolor A3(2) was nominated as a putative cellulase with 863 mature-form amino acids (90.58 kDa). We overexpressed and purified Sco6545 and demonstrated that the protein is not a cellulase but a xyloglucan-specific glycosyl hydrolase which cleaves xyloglucan at unbranched glucose residues.
Publisher
AMER SOC MICROBIOLOGY
Issue Date
2012-01
Language
English
Article Type
Article
Keywords

SUBSTRATE-SPECIFICITY; ASPERGILLUS-NIGER; EXPRESSION; ENDO-BETA-1,4-GLUCANASE; CLONING; ENDOGLUCANASES; CHRYSOSPORIUM; PURIFICATION; RECOGNITION; GENES

Citation

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, v.78, no.2, pp.607 - 611

ISSN
0099-2240
DOI
10.1128/AEM.06482-11
URI
http://hdl.handle.net/10203/94080
Appears in Collection
CBE-Journal Papers(저널논문)
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