DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Yeu-Chun | ko |
dc.contributor.author | Ludovice, Peter J. | ko |
dc.contributor.author | Prausnitz, Mark R. | ko |
dc.date.accessioned | 2013-03-08T14:36:42Z | - |
dc.date.available | 2013-03-08T14:36:42Z | - |
dc.date.created | 2012-06-21 | - |
dc.date.created | 2012-06-21 | - |
dc.date.issued | 2007-10 | - |
dc.identifier.citation | JOURNAL OF CONTROLLED RELEASE, v.122, pp.375 - 383 | - |
dc.identifier.issn | 0168-3659 | - |
dc.identifier.uri | http://hdl.handle.net/10203/93281 | - |
dc.description.abstract | In this study we tested the hypothesis that magainin, a peptide known to form pores in bacterial cell membranes, can increase skin penneability by disrupting stratum corneum lipid structure. We further hypothesized that magainin's enhancement requires co-administration with a surfactant chemical enhancer to increase magainin penetration into the skin. In support of these hypotheses, exposure to a known surfactant chemical enhancer, N-lauroyl sarcosine (NLS), in 50% ethanol solution increased in vitro skin permeability to fluorescein 15 fold and the combination of magainin and NLS-ethanol synergistically increased skin permeability 47 fold. In contrast, skin penneability was unaffected by exposure to magainin without co-enhancement by NLS-ethanol. Furthermore, confocal microscopy showed that magainin in the presence of NLS-ethanol penetrated deeply and extensively into stratum corneum, whereas magainin alone penetrated poorly into the skin. Additional analysis by Fourier-transform infrared spectroscopy, X-ray diffraction, and differential scanning calorimetry showed that NLS-ethanol disrupted stratum corneum lipid structure and that the combination of magainin and NLS-ethanol disrupted stratum corneum lipids even further. Altogether, these data suggest that NLS-ethanol increased magainin penetration into stratum corneum, which further increased stratum corneum lipid disruption and skin permeability. We believe this is the first study to demonstrate the use of a pore-forming peptide to increase skin permeability. This study also presents the novel concept of using a first chemical enhancer to increase penetration of a second chemical enhancer into the skin to synergistically increase skin permeability to a model drug. (C) 2007 Elsevier B.V. All rights reserved. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | STRATUM-CORNEUM LIPIDS | - |
dc.subject | X-RAY-DIFFRACTION | - |
dc.subject | HUMAN-SKIN | - |
dc.subject | DRUG-DELIVERY | - |
dc.subject | FATTY-ACIDS | - |
dc.subject | ANTIMICROBIAL PEPTIDES | - |
dc.subject | PROPYLENE-GLYCOL | - |
dc.subject | MECHANISM | - |
dc.subject | PENETRATION | - |
dc.subject | PERMEABILITY | - |
dc.title | Transdermal delivery enhanced by magainin pore-forming peptide | - |
dc.type | Article | - |
dc.identifier.wosid | 000250318800022 | - |
dc.identifier.scopusid | 2-s2.0-34548640798 | - |
dc.type.rims | ART | - |
dc.citation.volume | 122 | - |
dc.citation.beginningpage | 375 | - |
dc.citation.endingpage | 383 | - |
dc.citation.publicationname | JOURNAL OF CONTROLLED RELEASE | - |
dc.identifier.doi | 10.1016/j.jconrel.2007.05.031 | - |
dc.contributor.localauthor | Kim, Yeu-Chun | - |
dc.contributor.nonIdAuthor | Ludovice, Peter J. | - |
dc.contributor.nonIdAuthor | Prausnitz, Mark R. | - |
dc.type.journalArticle | Article; Proceedings Paper | - |
dc.subject.keywordAuthor | antimicrobial pore-fonning peptide | - |
dc.subject.keywordAuthor | magainin | - |
dc.subject.keywordAuthor | N-lauroylsarcosine | - |
dc.subject.keywordAuthor | Stratum corneum | - |
dc.subject.keywordAuthor | surfactant chemical enhancer | - |
dc.subject.keywordAuthor | transdermal drug delivery | - |
dc.subject.keywordPlus | STRATUM-CORNEUM LIPIDS | - |
dc.subject.keywordPlus | X-RAY-DIFFRACTION | - |
dc.subject.keywordPlus | HUMAN-SKIN | - |
dc.subject.keywordPlus | DRUG-DELIVERY | - |
dc.subject.keywordPlus | FATTY-ACIDS | - |
dc.subject.keywordPlus | ANTIMICROBIAL PEPTIDES | - |
dc.subject.keywordPlus | PROPYLENE-GLYCOL | - |
dc.subject.keywordPlus | MECHANISM | - |
dc.subject.keywordPlus | PENETRATION | - |
dc.subject.keywordPlus | PERMEABILITY | - |
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