Groundwater, used in this study, contaminated predominantly with aromatic compounds, was biologically treated in a fluidized-bed reactor (FBR) with immobilized cells. The aromatics were completely decomposed, while cis-1,2-dichloroethylene (cis-DCE) and trichloroethylene (TCE) were decomposed only approximately 20% and 5%, respectively. In these studies a significant improvement of the decomposition efficiency for chlorinated ethylenes was achieved by utilizing cometabolism. Methanol (MeOH) and toluene were used as the substrate in the case of one-stage reactor (Single Reactor). MeOH (187mgl(-1)) increased the decomposition efficiency up to 40% and 60% for cis-DCE and TCE, respectively, while toluene (20mgl(-1)) increased the decomposition efficiency of cis-DCE to 92% and the decomposition efficiency of TCE to 76%. In the case of two-stage reactor system (Reactor 1 and Reactor 2), MeOH and methane (CH4) were used as the substrate. In this system, cells grown on MeOH or CH4 in the Reactor 1 were continuously fed into Reactor 2 and groundwater was fed into Reactor 2 only. When MeOH (384mgl(-1)d(-1)) was used as substrate the decomposition efficiency of cis-DCE and TCE were 60% and 70%, respectively. Similar decomposition efficiency was observed for a small amount of CH4 (19.3mgl-(1)d-(1)). (C) 2004 Elsevier Ltd. All rights reserved.