Microvalve-assisted patterning platform for measuring cellular dynamics based on 3D cell culture

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A microfluidic platform to satisfy both 3D cell culture and cell-based assay is required for credible assay results and improved assay concept in drug discovery. In this article, we demonstrate a microvalve-assisted patterning (MAP) platform to provide a new method for investigating cellular dynamics by generating a linear concentration gradient of a drug as well as to realize 3D cell culture in a microenvironment. The MAP platform was fabricated by multilayer soft lithography and several microvalves made it possible to pattern a cell-matrix (scaffold) and to exchange media solutions without breaking cell-matrix structure in a microchannel. This approach provides not only exact fluids control, bubble removal, and stable solution exchange in a microchannel, but also reliable scaffold fabrication and 3D cell culture. In this study, hepatotoxicity tests with human hepatocellular liver carcinoma cells (HepG2) were also performed in real-time monitoring where cell morphologies exposed to different drug concentrations were observed at a time. Compared to 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, the MAP platform could be used to reduce drug amount and assay time for cell-based assays as much as 10 and 3 times, respectively.
Publisher
WILEY-BLACKWELL
Issue Date
2008-12
Language
English
Article Type
Article
Keywords

DRUG DISCOVERY; MICROFLUIDIC DEVICE; CYTOTOXICITY; SYSTEM; ASSAYS; ARRAY; ETHANOL

Citation

BIOTECHNOLOGY AND BIOENGINEERING, v.101, no.5, pp.1005 - 1013

ISSN
0006-3592
DOI
10.1002/bit.21962
URI
http://hdl.handle.net/10203/91816
Appears in Collection
BiS-Journal Papers(저널논문)
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