DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Minseok S. | ko |
dc.contributor.author | Lee, Won-Hye | ko |
dc.contributor.author | Kim, Yu-Chang | ko |
dc.contributor.author | Park, Je-Kyun | ko |
dc.date.accessioned | 2013-03-08T02:15:19Z | - |
dc.date.available | 2013-03-08T02:15:19Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2008-12 | - |
dc.identifier.citation | BIOTECHNOLOGY AND BIOENGINEERING, v.101, no.5, pp.1005 - 1013 | - |
dc.identifier.issn | 0006-3592 | - |
dc.identifier.uri | http://hdl.handle.net/10203/91816 | - |
dc.description.abstract | A microfluidic platform to satisfy both 3D cell culture and cell-based assay is required for credible assay results and improved assay concept in drug discovery. In this article, we demonstrate a microvalve-assisted patterning (MAP) platform to provide a new method for investigating cellular dynamics by generating a linear concentration gradient of a drug as well as to realize 3D cell culture in a microenvironment. The MAP platform was fabricated by multilayer soft lithography and several microvalves made it possible to pattern a cell-matrix (scaffold) and to exchange media solutions without breaking cell-matrix structure in a microchannel. This approach provides not only exact fluids control, bubble removal, and stable solution exchange in a microchannel, but also reliable scaffold fabrication and 3D cell culture. In this study, hepatotoxicity tests with human hepatocellular liver carcinoma cells (HepG2) were also performed in real-time monitoring where cell morphologies exposed to different drug concentrations were observed at a time. Compared to 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, the MAP platform could be used to reduce drug amount and assay time for cell-based assays as much as 10 and 3 times, respectively. | - |
dc.language | English | - |
dc.publisher | WILEY-BLACKWELL | - |
dc.subject | DRUG DISCOVERY | - |
dc.subject | MICROFLUIDIC DEVICE | - |
dc.subject | CYTOTOXICITY | - |
dc.subject | SYSTEM | - |
dc.subject | ASSAYS | - |
dc.subject | ARRAY | - |
dc.subject | ETHANOL | - |
dc.title | Microvalve-assisted patterning platform for measuring cellular dynamics based on 3D cell culture | - |
dc.type | Article | - |
dc.identifier.wosid | 000260949600015 | - |
dc.identifier.scopusid | 2-s2.0-56449113183 | - |
dc.type.rims | ART | - |
dc.citation.volume | 101 | - |
dc.citation.issue | 5 | - |
dc.citation.beginningpage | 1005 | - |
dc.citation.endingpage | 1013 | - |
dc.citation.publicationname | BIOTECHNOLOGY AND BIOENGINEERING | - |
dc.identifier.doi | 10.1002/bit.21962 | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Park, Je-Kyun | - |
dc.contributor.nonIdAuthor | Kim, Minseok S. | - |
dc.contributor.nonIdAuthor | Kim, Yu-Chang | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | microfluidic 3D cell culture | - |
dc.subject.keywordAuthor | cell-based assays | - |
dc.subject.keywordAuthor | multilayer soft lithography | - |
dc.subject.keywordAuthor | concentration gradient | - |
dc.subject.keywordPlus | DRUG DISCOVERY | - |
dc.subject.keywordPlus | MICROFLUIDIC DEVICE | - |
dc.subject.keywordPlus | CYTOTOXICITY | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | ASSAYS | - |
dc.subject.keywordPlus | ARRAY | - |
dc.subject.keywordPlus | ETHANOL | - |
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