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College of Engineering(공과대학)Dept. of Chemical and Biomolecular Engineering(생명화학공학과)CBE-Journal Papers(저널논문)

Beyond toothpicks: new methods for isolating mutant bacteria

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dc.contributor.authorLink, AJko
dc.contributor.authorJeong, Kijunko
dc.contributor.authorGeorgiou, Gko
dc.date.accessioned2013-03-07T15:56:52Z-
dc.date.available2013-03-07T15:56:52Z-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.issued2007-09-
dc.identifier.citationNATURE REVIEWS MICROBIOLOGY, v.5, no.9, pp.680 - 688-
dc.identifier.issn1740-1526-
dc.identifier.urihttp://hdl.handle.net/10203/90610-
dc.description.abstractOver the past 50 years genetic analysis in microbiology has relied predominantly on selections and plate assays using chromogenic enzyme substrates-for example, X-gal assays for the detection of beta-galactosidase activity. Recent advances in fluorescent assays and high throughput screening technologies have paved the way for the rapid isolation of mutants that confer complex phenotypes and for the quantitative analysis of the evolution of new traits in bacterial populations. This Review highlights the power of novel single-cell screening technologies and their applications to genetics, evolution and the biotechnological uses of bacteria.-
dc.languageEnglish-
dc.publisherNATURE PUBLISHING GROUP-
dc.subjectGREEN FLUORESCENT PROTEIN-
dc.subjectACTIVATED CELL SORTER-
dc.subjectESCHERICHIA-COLI-
dc.subjectDIRECTED EVOLUTION-
dc.subjectSURFACE-DISPLAY-
dc.subjectFLOW-CYTOMETRY-
dc.subjectPERIPLASMIC EXPRESSION-
dc.subjectPHYSIOLOGICAL-STATE-
dc.subjectMOLECULAR EVOLUTION-
dc.subjectANTIBODY FRAGMENTS-
dc.titleBeyond toothpicks: new methods for isolating mutant bacteria-
dc.typeArticle-
dc.identifier.wosid000248846500011-
dc.identifier.scopusid2-s2.0-34548032357-
dc.type.rimsART-
dc.citation.volume5-
dc.citation.issue9-
dc.citation.beginningpage680-
dc.citation.endingpage688-
dc.citation.publicationnameNATURE REVIEWS MICROBIOLOGY-
dc.identifier.doi10.1038/nrmicro1715-
dc.contributor.localauthorJeong, Kijun-
dc.contributor.nonIdAuthorLink, AJ-
dc.contributor.nonIdAuthorGeorgiou, G-
dc.type.journalArticleReview-
dc.subject.keywordPlusGREEN FLUORESCENT PROTEIN-
dc.subject.keywordPlusACTIVATED CELL SORTER-
dc.subject.keywordPlusESCHERICHIA-COLI-
dc.subject.keywordPlusDIRECTED EVOLUTION-
dc.subject.keywordPlusSURFACE-DISPLAY-
dc.subject.keywordPlusFLOW-CYTOMETRY-
dc.subject.keywordPlusPERIPLASMIC EXPRESSION-
dc.subject.keywordPlusPHYSIOLOGICAL-STATE-
dc.subject.keywordPlusMOLECULAR EVOLUTION-
dc.subject.keywordPlusANTIBODY FRAGMENTS-
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