Construction of asm2 deletion mutant of Actinosynnema pretiosum and medium optimization for ansamitocin P-3 production using statistical approach

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Ansamitocin P-3 is a potent antitumor agent produced by A. pretiosum. A deletion mutant of A. pretiosum was constructed by deleting the asm2 gene, a putative transcriptional repressor. The deletion mutant showed a 9-fold enhanced ansamitocin P-3 productivity. The response surface method with central composite design was employed to further optimize the culture medium composition for ansamitocin P-3) production by the deletion mutant. The concentrations of four medium ingredients, dextrin, maltose, cotton seed flour, and yeast extract, which have been reported as major components for ansamitocin production, were optimized through a series of flask culture experiments. The optimum concentrations of the selected factors were found to be dextrin 6.0%; maltose 3.0%; cotton seed flour 0.53%; and yeast extract 0.45%. The maximum titer of ansamitocin P-3 was 78.3mg/l with the optimized composition, about 15-folds higher than the unoptimized titer of 5.0 mg/l obtained with YMG medium.
Publisher
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
Issue Date
2006-09
Language
English
Article Type
Article
Keywords

RESPONSE-SURFACE METHODOLOGY; ANTITUMOR AGENT ANSAMITOCIN; CHAIN EXTENSION UNIT; SACCHAROMYCES-CEREVISIAE; ACTINORHODIN PRODUCTION; STREPTOMYCES-PEUCETIUS; CULTURE-MEDIUM; BIOSYNTHESIS; GENE; IDENTIFICATION

Citation

JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.16, no.9, pp.1338 - 1346

ISSN
1017-7825
URI
http://hdl.handle.net/10203/89888
Appears in Collection
CBE-Journal Papers(저널논문)
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