An inducible translocation strategy to rapidly activate and inhibit small GTPase signaling pathways

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We made substantial advances in the implementation of a rapa mycin-triggered heterodimerization strategy. Using molecular engineering of different targeting and enzymatic fusion constructs and a new rapamycin analog, Rho GTPases were directly activated or inactivated on a timescale of seconds, which was followed by pronounced cell morphological changes. As signaling processes often occur within minutes, such rapid perturbations provide a powerful tool to investigate the role, selectivity and timing of Rho GTPase-mediated signaling processes.
Publisher
NATURE PUBLISHING GROUP
Issue Date
2005-06
Language
English
Article Type
Article
Keywords

ACTIN POLYMERIZATION; MEMBRANE RECRUITMENT; BINDING PROTEINS; KINASE; RAC; DOMAIN

Citation

NATURE METHODS, v.2, no.6, pp.415 - 418

ISSN
1548-7091
DOI
10.1038/NMETH763
URI
http://hdl.handle.net/10203/89142
Appears in Collection
BS-Journal Papers(저널논문)
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