Effects of supplementation of various medium components on Chinese hamster ovary cell cultures producing recombinant antibody

Cited 27 time in webofscience Cited 0 time in scopus
  • Hit : 265
  • Download : 0
Thirteen vitamins, twenty amino acids, hormones, inorganic salts, and other chemical agents, which constitute typical serum-free media, were evaluated for the development of fortified medium to enhance cell growth and productivity of recombinant antibody in the cultures of the recombinant Chinese hamster ovary (rCHO) cells. Two different rCHO cell lines, rCHO-A producing recombinant antibodies against the human platelet and rCHO-B secreting recombinant antibodies against the S surface antigen of Hepatitis B, respectively, were cultivated in batch suspension mode. Concentration of interested component in the tested medium was doubled to examine the fortification effect. Growth of rCHO-A cell and its antibody production were slightly improved with addition of either choline chloride, folic acid, thiamine(.)HCl, or Long (TM) R(3)IGF-I. On the other hand, in the cultivation of rCHO-B cell which was more sensitive to its environmental changes, hormones such as Long (TM) R(3)IGF-I and triiodothyronine (T-3) as well as various vitamins involving choline chloride, i-inositol, niacinamide, pyridoxine HCl, and thiamine(.)HCl enhanced the cell growth and antibody production. Particularly, when concentration of consuming amino acid was doubled, remarkable increase in specific productivity was served, resulting in high final antibody concentration. These results were believed to provide a fundamental strategy of medium fortification useful for improvement of recombinant antibody production in serum-free medium.
Publisher
Springer
Issue Date
2005-01
Language
English
Article Type
Article; Proceedings Paper
Keywords

SERUM-FREE MEDIUM; FILTER PERFUSION SYSTEM; PROTEIN-FREE MEDIUM; BETA-MERCAPTOETHANOL; SUSPENSION-CULTURE; HYBRIDOMA GROWTH; AMINO-ACIDS; INSULIN; TRANSFERRIN; IMPROVEMENT

Citation

CYTOTECHNOLOGY, v.47, no.1-3, pp.37 - 49

ISSN
0920-9069
URI
http://hdl.handle.net/10203/89082
Appears in Collection
CBE-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 27 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0