DC Field | Value | Language |
---|---|---|
dc.contributor.author | Beyor, Nathaniel | ko |
dc.contributor.author | SEO, TAE SEOK | ko |
dc.contributor.author | Liu, Peng | ko |
dc.contributor.author | Mathies, Richard A. | ko |
dc.date.accessioned | 2009-05-06T05:47:48Z | - |
dc.date.available | 2009-05-06T05:47:48Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2008-12 | - |
dc.identifier.citation | BIOMEDICAL MICRODEVICES, v.10, no.6, pp.909 - 917 | - |
dc.identifier.issn | 1387-2176 | - |
dc.identifier.uri | http://hdl.handle.net/10203/8863 | - |
dc.description.abstract | A cell concentration microdevice for immunomagnetic pathogen isolation from a dilute sample is presented. Cells are driven by integrated on-chip pumps through a fluidized bed of immobilized immunomagnetic beads. Off-chip polymerase chain reaction and capillary electrophoretic analysis are used to determine capture efficiencies of E. coli and to optimize the system. Beads are immobilized after each split in a bifurcated channel system to ensure a balanced distribution of beads in all the capture channels. The addition of a pumping flutter step to repeatedly drive sample through the bead bed was found to enhance capture. Capture efficiencies of 70% and a limit of detection of 2 cfu/mu L were achieved; specific capture of E. coli at a concentration of 100 cfu/mu L in a 100-fold background of S. aureus is shown. This capture/concentration system is an important step in overcoming the macro-to-micro interface challenge in the development of microdevices for pathogen detection. | - |
dc.description.sponsorship | the NIH (#U01AI056472) | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | SPRINGER | - |
dc.subject | SOLID-PHASE EXTRACTION | - |
dc.subject | MICROFLUIDIC DEVICE | - |
dc.subject | CAPILLARY ELECTROPHORESIS | - |
dc.subject | SAMPLE PREPARATION | - |
dc.subject | DNA | - |
dc.subject | SEPARATION | - |
dc.subject | SYSTEMS | - |
dc.subject | BLOOD | - |
dc.subject | AMPLIFICATION | - |
dc.subject | BIOPROCESSOR | - |
dc.title | Immunomagnetic bead-based cell concentration microdevice for dilute pathogen detection | - |
dc.type | Article | - |
dc.identifier.wosid | 000259246400015 | - |
dc.identifier.scopusid | 2-s2.0-52449127957 | - |
dc.type.rims | ART | - |
dc.citation.volume | 10 | - |
dc.citation.issue | 6 | - |
dc.citation.beginningpage | 909 | - |
dc.citation.endingpage | 917 | - |
dc.citation.publicationname | BIOMEDICAL MICRODEVICES | - |
dc.identifier.doi | 10.1007/s10544-008-9206-3 | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | SEO, TAE SEOK | - |
dc.contributor.nonIdAuthor | Beyor, Nathaniel | - |
dc.contributor.nonIdAuthor | Liu, Peng | - |
dc.contributor.nonIdAuthor | Mathies, Richard A. | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | pathogen detection | - |
dc.subject.keywordAuthor | microfluidic | - |
dc.subject.keywordAuthor | lab-on-a-chip | - |
dc.subject.keywordAuthor | sample preconcentration | - |
dc.subject.keywordAuthor | immunomagnetic bead capture | - |
dc.subject.keywordPlus | SOLID-PHASE EXTRACTION | - |
dc.subject.keywordPlus | MICROFLUIDIC DEVICE | - |
dc.subject.keywordPlus | CAPILLARY ELECTROPHORESIS | - |
dc.subject.keywordPlus | SAMPLE PREPARATION | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | SEPARATION | - |
dc.subject.keywordPlus | SYSTEMS | - |
dc.subject.keywordPlus | BLOOD | - |
dc.subject.keywordPlus | AMPLIFICATION | - |
dc.subject.keywordPlus | BIOPROCESSOR | - |
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